Abstract
We have devised a method of temperature scanning with a vibrating-U-tube density meter in which temperature fluctuations are much reduced compared to those using a constant or programmable thermostat. The standard error of a density measurement is 5 × 10-7 g/ml. Volume changes associated with conformational changes of macromolecular systems can be precisely measured. Using this instrument the volume expansion-melting curves of lipid dispersions have been obtained. The curves are similar in shape and resolution to the excess heat-capacity curves derived from differential scanning calorimetry performed on the same sample. Temperature scanning allows measurements of expansivity as well as apparent volume throughout a temperature range of interest.
Original language | English (US) |
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Pages (from-to) | 161-167 |
Number of pages | 7 |
Journal | Analytical biochemistry |
Volume | 116 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1 1981 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology