Modest overexpression of FOXO maintains cardiac proteostasis and ameliorates age-associated functional decline

Anna C. Blice-Baum, Alexander C. Zambon, Gaurav Kaushik, Meera C. Viswanathan, Adam J. Engler, Rolf Bodmer, Anthony Cammarato

Research output: Contribution to journalArticlepeer-review

Abstract

Heart performance declines with age. Impaired protein quality control (PQC), due to reduced ubiquitin-proteasome system (UPS) activity, autophagic function, and/or chaperone-mediated protein refolding, contributes to cardiac deterioration. The transcription factor FOXO participates in regulating genes involved in PQC, senescence, and numerous other processes. Here, a comprehensive approach, involving molecular genetics, novel assays to probe insect cardiac physiology, and bioinformatics, was utilized to investigate the influence of heart-restricted manipulation of dFOXO expression in the rapidly aging Drosophila melanogaster model. Modest dFOXO overexpression was cardioprotective, ameliorating nonpathological functional decline with age. This was accompanied by increased expression of genes associated predominantly with the UPS, relative to other PQC components, which was validated by a significant decrease in ubiquitinated proteins. RNAi knockdown of UPS candidates accordingly compromised myocardial physiology in young flies. Conversely, excessive dFOXO overexpression or suppression proved detrimental to heart function and/or organismal development. This study highlights D. melanogaster as a model of cardiac aging and FOXO as a tightly regulated mediator of proteostasis and heart performance over time.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalAging Cell
Volume16
Issue number1
DOIs
StatePublished - Feb 1 2017

Keywords

  • Drosophila
  • FOXO
  • UPS
  • autophagy
  • cardiac aging
  • protein homeostasis

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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