Models of development of nonreceptor methods for distinguishing androgen-sensitive and -insensitive prostatic tumors

Research output: Contribution to journalArticle

Abstract

From the original Dunning R-3327 rat prostatic adenocarcinoma, several distinct sublines have been obtained. These sublines include a well-differentiated, slow-growing, androgen-sensitive tumor (R-3327-H); a well-differentiated, slow-growing, androgen-insensitivie tumor (R-3327-HI); and a fast-growing, androgen-insensitive, anaplastic tumor (R3327-AT). These three sublines were compared in order to develop new model methods for the prediction of the androgen sensitivity and the degree of differentiation of prostatic adenocarcinomas. The R-3327-AT was very distinct in all parameters examined except the tissue protein electrophoretic patterns which contained a uniform pattern in all tumors. The significant differences between R-3327-H and -HI sublines were (a) the inability of testosterone of stimulate DNA synthesis in the R-3327-HI tumor and (b) the difference in the enzymatic profiles of these sublines. The specific activity of three enzymes (3α-hydroxysteroid dehydrogenase, leucine aminopeptidase, lactic dehydrogenase) increased while the activity of another three enzymes (6α, 7α-hydroxylase, 5α-reductase, alkaline phosphatase) decreased in the sublines which are androgen insensitive and less differentiated. A arbitrary index was constructed, based upon these enzyme differences, which clearly discriminates the degree of androgen sensitivity and differentation of these R-3327 rat prostatic adenocarcinomas.

Original languageEnglish (US)
Pages (from-to)2652-2659
Number of pages8
JournalCancer Research
Volume39
Issue number7 I
StatePublished - 1979

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Androgens
Neoplasms
Adenocarcinoma
Oxidoreductases
Enzymes
3-Hydroxysteroid Dehydrogenases
Leucyl Aminopeptidase
Mixed Function Oxygenases
Alkaline Phosphatase
Testosterone
Milk
DNA
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Models of development of nonreceptor methods for distinguishing androgen-sensitive and -insensitive prostatic tumors. / Isaacs, John Tod; Isaacs, William B; Coffey, D. S.

In: Cancer Research, Vol. 39, No. 7 I, 1979, p. 2652-2659.

Research output: Contribution to journalArticle

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