@article{43dd8060fedf4368b24031861ed218c3,
title = "Modeling a genetic risk for schizophrenia in iPSCs and Mice reveals neural stem cell deficits associated with adherens junctions and polarity",
abstract = "Defects in brain development are believed to contribute toward the onset of neuropsychiatric disorders, but identifying specific underlying mechanisms has proven difficult. Here, we took a multifaceted approach to investigate why 15q11.2 copy number variants are prominent risk factors for schizophrenia and autism. First, we show that human iPSC-derived neural progenitors carrying 15q11.2 microdeletion exhibit deficits in adherens junctions and apical polarity. This results from haploinsufficiency of CYFIP1, a gene within 15q11.2 that encodes a subunit of the WAVE complex, which regulates cytoskeletal dynamics. In developing mouse cortex, deficiency in CYFIP1 and WAVE signaling similarly affects radial glial cells, leading to their ectopic localization outside of the ventricular zone. Finally, targeted human genetic association analyses revealed an epistatic interaction between CYFIP1 and WAVE signaling mediator ACTR2 and risk for schizophrenia. Our findings provide insight into how CYFIP1 regulates neural stem cell function and may contribute to the susceptibility of neuropsychiatric disorders.",
author = "Yoon, {Ki Jun} and Nguyen, {Ha Nam} and Gianluca Ursini and Fengyu Zhang and Kim, {Nam Shik} and Zhexing Wen and Georgia Makri and David Nauen and Shin, {Joo Heon} and Youngbin Park and Raeeun Chung and Eva Pekle and Ce Zhang and Maxwell Towe and Hussaini, {Syed Mohammed Qasim} and Yohan Lee and Dan Rujescu and {St. Clair}, David and Kleinman, {Joel E.} and Hyde, {Thomas M.} and Gregory Krauss and Christian, {Kimberly M.} and Rapoport, {Judith L.} and Weinberger, {Daniel R.} and Hongjun Song and Ming, {Guo Li}",
note = "Funding Information: We would like to thank members of G.-l.M. and H.S. laboratories for discussion, ICE stem cell core and H. Kim for generating some iPSC lines, K. Ahn, T. Andersen, V. Villagomez, L. Liu, and Y. Cai for technical support and help. This work was supported by NIH (NS048271, HD069184), the Brain and Behavior Research Foundation (NARSAD), and the Maryland Stem Cell Research Fund (MSCRF) (to G.-l.M.), the Simons Foundation Autism Research Initiative (SFARI), NIH (NS047344, MH087874), and the International Mental Health Research Organization (IMHRO) to H.S., the Lieber Institute for Brain Development to D.R.W., J.E.K., and T.M.H., NARSAD and MSCRF to K.M.C., by fellowships from HFSP to K-j.Y. and MSCRF to G.M., N.-S.K., and Z.W., and from NIH (F31MH102978) to H.N.N. ",
year = "2014",
month = jul,
day = "3",
doi = "10.1016/j.stem.2014.05.003",
language = "English (US)",
volume = "15",
pages = "79--91",
journal = "Cell stem cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "1",
}