Mixed-mode electrokinetic and chromatographic peptide separations in a microvalve-integrated polymer chip

Jikun Liu, Chien Fu Chen, Shuang Yang, Chien Cheng Chang, Don L. Devoe

Research output: Contribution to journalArticlepeer-review

Abstract

A cycloolefin polymer chip supporting the concatenation of isoelectric focusing (IEF) and reversed-phase liquid chromatography (RPLC) is demonstrated for high throughput two dimensional peptide separations. A unique benefit of the mixed-mode platform is the ability of IEF to act as a highly concentrating electrokinetic separation mode for effective isolation of sample components prior to RPLC. The thermoplastic chip contains integrated high pressure microvalves, enabling uniform sample transfer from the IEF channel to multiple parallel RPLC channels, gradient elution from each RPLC column, and hydrodynamic isolation between the separation dimensions. The reusable system is shown to provide efficient 2-D separations together with facile interfacing with MALDI-MS, suggesting a new path towards effective peptide analysis from complex samples.

Original languageEnglish (US)
Pages (from-to)2122-2129
Number of pages8
JournalLab on a Chip
Volume10
Issue number16
DOIs
StatePublished - Aug 21 2010

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Mixed-mode electrokinetic and chromatographic peptide separations in a microvalve-integrated polymer chip'. Together they form a unique fingerprint.

Cite this