Abstract
Happy TRAILs to you: PEGylation of proteins through complementary interactions between a His-tag and a Ni2+ complex of nitrilotriacetic acid (NTA, see picture), a well-established practice in protein research, was used to improve the half-life of therapeutic proteins in the blood following systemic administration in vivo. Animal models show that this site-specific modification improves the efficacy of modified TRAIL proteins.
Original language | English (US) |
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Pages (from-to) | 6880-6884 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 52 |
Issue number | 27 |
DOIs | |
State | Published - Jul 1 2013 |
Keywords
- PEGylation
- drug screening
- histidine tags
- protein design
- protein modifications
ASJC Scopus subject areas
- Catalysis
- General Chemistry