Mitotic membrane helps to focus and stabilize the mitotic spindle

Christopher C. Poirier; Yixian Zheng; Pablo A. Iglesias

doi:10.1016/j.bpj.2010.09.053

Mitotic membrane helps to focus and stabilize the mitotic spindle

Christopher C. Poirier, Yixian Zheng, Pablo A. Iglesias

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

During mitosis, microtubules (MTs), aided by motors and associated proteins, assemble into a mitotic spindle. Recent evidence supports the notion that a membranous spindle matrix aids spindle formation; however, the mechanisms by which the matrix may contribute to spindle assembly are unknown. To search for a mechanism by which the presence of a mitotic membrane might help spindle morphology, we built a computational model that explores the interactions between these components. We show that an elastic membrane around the mitotic apparatus helps to focus MT minus ends and provides a resistive force that acts antagonistically to plus-end-directed MT motors such as Eg5.

Original language	English (US)
Pages (from-to)	3182-3190
Number of pages	9
Journal	Biophysical journal
Volume	99
Issue number	10
DOIs	https://doi.org/10.1016/j.bpj.2010.09.053
State	Published - Nov 17 2010

ASJC Scopus subject areas

Biophysics

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10.1016/j.bpj.2010.09.053

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title = "Mitotic membrane helps to focus and stabilize the mitotic spindle",

abstract = "During mitosis, microtubules (MTs), aided by motors and associated proteins, assemble into a mitotic spindle. Recent evidence supports the notion that a membranous spindle matrix aids spindle formation; however, the mechanisms by which the matrix may contribute to spindle assembly are unknown. To search for a mechanism by which the presence of a mitotic membrane might help spindle morphology, we built a computational model that explores the interactions between these components. We show that an elastic membrane around the mitotic apparatus helps to focus MT minus ends and provides a resistive force that acts antagonistically to plus-end-directed MT motors such as Eg5.",

author = "Poirier, {Christopher C.} and Yixian Zheng and Iglesias, {Pablo A.}",

note = "Funding Information: This work was supported in part by the National Institutes of Health (GM056312 to Y.X. and GM086704 to P.A.I.) and the National Science Foundation (0621740 to P.A.I.). Y.Z. is an investigator of the Howard Hughes Medical Institute. ",

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T1 - Mitotic membrane helps to focus and stabilize the mitotic spindle

AU - Poirier, Christopher C.

AU - Zheng, Yixian

AU - Iglesias, Pablo A.

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N2 - During mitosis, microtubules (MTs), aided by motors and associated proteins, assemble into a mitotic spindle. Recent evidence supports the notion that a membranous spindle matrix aids spindle formation; however, the mechanisms by which the matrix may contribute to spindle assembly are unknown. To search for a mechanism by which the presence of a mitotic membrane might help spindle morphology, we built a computational model that explores the interactions between these components. We show that an elastic membrane around the mitotic apparatus helps to focus MT minus ends and provides a resistive force that acts antagonistically to plus-end-directed MT motors such as Eg5.

AB - During mitosis, microtubules (MTs), aided by motors and associated proteins, assemble into a mitotic spindle. Recent evidence supports the notion that a membranous spindle matrix aids spindle formation; however, the mechanisms by which the matrix may contribute to spindle assembly are unknown. To search for a mechanism by which the presence of a mitotic membrane might help spindle morphology, we built a computational model that explores the interactions between these components. We show that an elastic membrane around the mitotic apparatus helps to focus MT minus ends and provides a resistive force that acts antagonistically to plus-end-directed MT motors such as Eg5.

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Mitotic membrane helps to focus and stabilize the mitotic spindle

Abstract

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