Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1

Megan L. Povelones, Calvin Tiengwe, Eva Gluenz, Keith Gull, Paul T. Englund, Robert E. Jensen

Research output: Contribution to journalArticlepeer-review

Abstract

In an RNAi library screen for loss of kinetoplast DNA (kDNA), we identified an uncharacterized Trypanosoma brucei protein, named TbLOK1, required for maintenance of mitochondrial shape and function. We found the TbLOK1 protein located in discrete patches in the mitochondrial outer membrane. Knock-down of TbLOK1 in procyclic trypanosomes caused the highly interconnected mitochondrial structure to collapse, forming an unbranched tubule remarkably similar to the streamlined organelle seen in the bloodstream form. Following RNAi, defects in mitochondrial respiration, inner membrane potential and mitochondrial transcription were observed. At later times following TbLOK1 depletion, kDNA was lost and a more drastic alteration in mitochondrial structure was found. Our results demonstrate the close relationship between organelle structure and function in trypanosomes.

Original languageEnglish (US)
Pages (from-to)713-729
Number of pages17
JournalMolecular Microbiology
Volume87
Issue number4
DOIs
StatePublished - Feb 2013
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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