RATIONALE:: Activation of the mitochondrial ATP-sensitive potassium channel (mitoKATP) has been impliCated in the mechanism of Cardiac ischemic preconditioning, yet its molecular composition is unknown. OBJECTIVE:: To use an unbiased proteomic analysis of the mitochondrial inner membrane to identify the mitochondrial K channel underlying mitoKATP. METHODS AND RESULTS:: Mass spectrometric analysis was used to identify KCNJ1(ROMK) in purified bovine heart mitochondrial inner membrane and ROMK mRNA was confirmed to be present in neonatal rat ventricular myo cytes and adult hearts. ROMK2, a short form of the channel, is shown to contain an N-terminal mitochondrial targeting signal, and a full-length epitope-tagged ROMK2 coloCalizes with mitochondrial ATP synthase β. The high-affinity ROMK toxin, tertiapin Q, inhibits mitoKATP activity in isolated mitochondria and in digitonin-permeabilized cells. Moreover, short hairpin RNA-mediated knockdown of ROMK inhibits the ATP-sensitive, diazoxide-activated component of mitochondrial thallium uptake. Finally, the heart-derived cell line, H9C2, is protected from cell death stimuli by stable ROMK2 overexpression, whereas knockdown of the native ROMK exacerbates cell death. CONCLUSIONS:: The findings support ROMK as the pore-forming subunit of the cytoprotective mitoKATP channel.
- ATP-sensitive potassium channel
- renal outer medullary potassium channel
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine