Mitochondrial Function in Human Neuroblastoma Cells Is Up-Regulated and Protected by NQO1, a Plasma Membrane Redox Enzyme

Jiyeong Kim, Su Kyung Kim, Hwa Kyung Kim, Mark P. Mattson, Dong Hoon Hyun

Research output: Contribution to journalArticle

Abstract

Background:Recent findings suggest that NADH-dependent enzymes of the plasma membrane redox system (PMRS) play roles in the maintenance of cell bioenergetics and oxidative state. Neurons and tumor cells exhibit differential vulnerability to oxidative and metabolic stress, with important implications for the development of therapeutic interventions that promote either cell survival (neurons) or death (cancer cells).Methods and Findings:Here we used human neuroblastoma cells with low or high levels of the PMRS enzyme NADH-quinone oxidoreductase 1 (NQO1) to investigate how the PMRS modulates mitochondrial functions and cell survival. Cells with elevated NQO1 levels exhibited higher levels of oxygen consumption and ATP production, and lower production of reactive oxygen species. Cells overexpressing NQO1 were more resistant to being damaged by the mitochondrial toxins rotenone and antimycin A, and exhibited less oxidative/nitrative damage and less apoptotic cell death. Cells with basal levels of NQO1 resulted in increased oxidative damage to proteins and cellular vulnerability to mitochondrial toxins. Thus, mitochondrial functions are enhanced and oxidative stress is reduced as a result of elevated PMRS activity, enabling cells to maintain redox homeostasis under conditions of metabolic and energetic stress.Conclusion:These findings suggest that NQO1 is a potential target for the development of therapeutic agents for either preventing neuronal degeneration or promoting the death of neural tumor cells.

Original languageEnglish (US)
Article numbere69030
JournalPLoS One
Volume8
Issue number7
DOIs
StatePublished - Jul 11 2013
Externally publishedYes

Fingerprint

oxidoreductases
Cell membranes
quinones
Neuroblastoma
Oxidation-Reduction
plasma membrane
Cells
Cell Membrane
Enzymes
enzymes
Neurons
cells
Tumors
cell viability
Antimycin A
toxins
Rotenone
neurons
Oxidative stress
death

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mitochondrial Function in Human Neuroblastoma Cells Is Up-Regulated and Protected by NQO1, a Plasma Membrane Redox Enzyme. / Kim, Jiyeong; Kim, Su Kyung; Kim, Hwa Kyung; Mattson, Mark P.; Hyun, Dong Hoon.

In: PLoS One, Vol. 8, No. 7, e69030, 11.07.2013.

Research output: Contribution to journalArticle

Kim, Jiyeong ; Kim, Su Kyung ; Kim, Hwa Kyung ; Mattson, Mark P. ; Hyun, Dong Hoon. / Mitochondrial Function in Human Neuroblastoma Cells Is Up-Regulated and Protected by NQO1, a Plasma Membrane Redox Enzyme. In: PLoS One. 2013 ; Vol. 8, No. 7.
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