Mitochondrial ATP synthase: Quaternary structure of the F1 moiety at 3.6 Å determined by x-ray diffraction analysis

Mario Antonio Bianchet, Xavier Ysern, Joanne Hullihen, Peter L Pedersen, Mario L Amzel

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Abstract

The F1 moiety of the mitochondrial ATP synthase is composed of five different subunits with stoichiometry α3β3γδε and exhibits the capacity to synthesize ATP from ADP and Pi. We have previously crystallized rat liver F1 and described its structure at 9-Å resolution (Amzel, L. M., McKinney, M., Narayanan, P., and Pedersen, P. L. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 5852-5856). Here we present an x-ray map of this complex enzyme at 3.6 Å, which provides a much more informative description of its quaternary structure. The overall dimensions of the F1 molecule are 120 Å × 120 Å × 74 Å. The enzyme exhibits 3-fold symmetry relating the three copies of each of the two major subunits, α and β. As the α subunits (but not the β subunits) contain cysteine residues, it has been possible to identify the α subunits by heavy atom labeling with mersalyl and to relate their positions in the F1 molecule to the β subunits. Significantly, the α and β subunits each exist as trimeric arrays which are organized in two slightly offset, interdigitated layers along the 3-fold axis. In one trimeric layer the α subunits are located close to the axis with homologous subunits interacting with each other; in the other trimeric layer the β subunits are far from the axis, and they interact only with α subunits and not with one another. At one end of the structure, part of the interface between each α and β subunit encloses a space or "pocket" that is accessible to the solvent; at the other end the interfaces between the subunits are more open and exposed. The present work represents the highest resolution map reported to date for the F1 moiety of an ATP synthase complex.

Original languageEnglish (US)
Pages (from-to)21197-21201
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number31
StatePublished - Nov 5 1991

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Mitochondrial Proton-Translocating ATPases
Mersalyl
Adenosine Triphosphate
Diffraction
X-Rays
X rays
Molecules
Enzymes
Stoichiometry
Liver
Adenosine Diphosphate
Labeling
Cysteine
Rats
Atoms

ASJC Scopus subject areas

  • Biochemistry

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Mitochondrial ATP synthase : Quaternary structure of the F1 moiety at 3.6 Å determined by x-ray diffraction analysis. / Bianchet, Mario Antonio; Ysern, Xavier; Hullihen, Joanne; Pedersen, Peter L; Amzel, Mario L.

In: Journal of Biological Chemistry, Vol. 266, No. 31, 05.11.1991, p. 21197-21201.

Research output: Contribution to journalArticle

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abstract = "The F1 moiety of the mitochondrial ATP synthase is composed of five different subunits with stoichiometry α3β3γδε and exhibits the capacity to synthesize ATP from ADP and Pi. We have previously crystallized rat liver F1 and described its structure at 9-{\AA} resolution (Amzel, L. M., McKinney, M., Narayanan, P., and Pedersen, P. L. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 5852-5856). Here we present an x-ray map of this complex enzyme at 3.6 {\AA}, which provides a much more informative description of its quaternary structure. The overall dimensions of the F1 molecule are 120 {\AA} × 120 {\AA} × 74 {\AA}. The enzyme exhibits 3-fold symmetry relating the three copies of each of the two major subunits, α and β. As the α subunits (but not the β subunits) contain cysteine residues, it has been possible to identify the α subunits by heavy atom labeling with mersalyl and to relate their positions in the F1 molecule to the β subunits. Significantly, the α and β subunits each exist as trimeric arrays which are organized in two slightly offset, interdigitated layers along the 3-fold axis. In one trimeric layer the α subunits are located close to the axis with homologous subunits interacting with each other; in the other trimeric layer the β subunits are far from the axis, and they interact only with α subunits and not with one another. At one end of the structure, part of the interface between each α and β subunit encloses a space or {"}pocket{"} that is accessible to the solvent; at the other end the interfaces between the subunits are more open and exposed. The present work represents the highest resolution map reported to date for the F1 moiety of an ATP synthase complex.",
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