Mitochondrial ATP synthase: A bioinformatic approach reveals new insights about the roles of supernumerary subunits g and A6L

Sangjin Hong, Peter L Pedersen

Research output: Contribution to journalArticle

Abstract

The mitochondrial ATP synthase is a membrane protein complex which couples the proton gradient across the mitochondrial inner membrane to the synthesis of ATP from ADP + Pi. The complex is composed of essential subunits for its motor functions and supernumerary subunits, the roles of which remain to be elucidated. Subunits g and A6L are supernumerary subunits, and the specific roles of these subunits are still matters of debate. To gain insight into the functions of these two subunits, we carried out the alignment and the homolog search of the protein sequences of the subunits and found the following features: Subunit g appears to have isoforms in animals, and the transmembrane domain of the animal subunit g contains a completely conserved acidic residue in the middle of a helix on the conserved side of the transmembrane helix. This finding implicates the conserved acidic residue as important for the function of subunit g. The alignment of A6L protein sequences shows a conserved aromatic residue at the N-terminal domain with which the N-terminal MPQL sequence comprises a unique MPQLX4Ar motif that can signify the protein A6L. The conserved aromatic residue may also be important for the function of A6L.

Original languageEnglish (US)
Pages (from-to)515-523
Number of pages9
JournalJournal of Bioenergetics and Biomembranes
Volume36
Issue number6
DOIs
StatePublished - Dec 2004

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Mitochondrial Proton-Translocating ATPases
Computational Biology
Protein Subunits
Mitochondrial Membranes
Adenosine Diphosphate
Protons
Protein Isoforms
Membrane Proteins
Proteins
Adenosine Triphosphate

Keywords

  • ATP synthase
  • Bioinformatics
  • Mitochondria
  • Subunit A6L
  • Subunit g
  • Supernumerary subunits

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Mitochondrial ATP synthase : A bioinformatic approach reveals new insights about the roles of supernumerary subunits g and A6L. / Hong, Sangjin; Pedersen, Peter L.

In: Journal of Bioenergetics and Biomembranes, Vol. 36, No. 6, 12.2004, p. 515-523.

Research output: Contribution to journalArticle

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