Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking

Tinghan Zhao, Xinglei Liu, Sweety Singh, Xiangshan Liu, Yuanwei Zhang, Junko Sawada, Masanobu Komatsu, Kevin D. Belfield

Research output: Contribution to journalArticle

Abstract

Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.

Original languageEnglish (US)
JournalBioconjugate Chemistry
DOIs
StateAccepted/In press - Jan 1 2019

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Mitochondria
Phenylalanine
Peptides
Neurodegenerative diseases
Mitochondrial Diseases
Rhodamines
Fluorescence microscopy
Mitochondrial Membranes
Chromophores
Cytotoxicity
Medical problems
Biocompatibility
Fluorescence Microscopy
Oxygen Consumption
Neurodegenerative Diseases
Contrast Media
Amino acids
Assays
Cell Survival
Cardiovascular Diseases

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking. / Zhao, Tinghan; Liu, Xinglei; Singh, Sweety; Liu, Xiangshan; Zhang, Yuanwei; Sawada, Junko; Komatsu, Masanobu; Belfield, Kevin D.

In: Bioconjugate Chemistry, 01.01.2019.

Research output: Contribution to journalArticle

Zhao, Tinghan ; Liu, Xinglei ; Singh, Sweety ; Liu, Xiangshan ; Zhang, Yuanwei ; Sawada, Junko ; Komatsu, Masanobu ; Belfield, Kevin D. / Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking. In: Bioconjugate Chemistry. 2019.
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AU - Sawada, Junko

AU - Komatsu, Masanobu

AU - Belfield, Kevin D.

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