Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking

Tinghan Zhao; Xinglei Liu; Sweety Singh; Xiangshan Liu; Yuanwei Zhang; Junko Sawada; Masanobu Komatsu; Kevin D. Belfield

doi:10.1021/acs.bioconjchem.9b00465

Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking

Tinghan Zhao, Xinglei Liu, Sweety Singh, Xiangshan Liu, Yuanwei Zhang, Junko Sawada, Masanobu Komatsu, Kevin D. Belfield

All Children's Hospital

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH₂ (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.

Original language	English (US)
Pages (from-to)	2312-2316
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	30
Issue number	9
DOIs	https://doi.org/10.1021/acs.bioconjchem.9b00465
State	Published - Sep 18 2019

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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title = "Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking",

abstract = "Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.",

author = "Tinghan Zhao and Xinglei Liu and Sweety Singh and Xiangshan Liu and Yuanwei Zhang and Junko Sawada and Masanobu Komatsu and Belfield, {Kevin D.}",

note = "Funding Information: We wish to acknowledge support from the National Science Foundation (CBET-1517273, CBET-1403535, and CHE-1726345). KDB wishes to acknowledge support from the Becton-Dickinson Research Professorship. Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",

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doi = "10.1021/acs.bioconjchem.9b00465",

language = "English (US)",

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AU - Zhao, Tinghan

AU - Liu, Xinglei

AU - Singh, Sweety

AU - Liu, Xiangshan

AU - Zhang, Yuanwei

AU - Sawada, Junko

AU - Komatsu, Masanobu

AU - Belfield, Kevin D.

N1 - Funding Information: We wish to acknowledge support from the National Science Foundation (CBET-1517273, CBET-1403535, and CHE-1726345). KDB wishes to acknowledge support from the Becton-Dickinson Research Professorship. Publisher Copyright: Copyright © 2019 American Chemical Society.

PY - 2019/9/18

Y1 - 2019/9/18

N2 - Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.

AB - Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.

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Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking

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