miRNA profiles of monocyte-lineage cells are consistent with complicated roles in HIV-1 restriction

Research output: Contribution to journalArticle

Abstract

Long-lived HIV-1 reservoirs include tissue macrophages. Monocyte-derived macrophages are more susceptible to infection and more permissive to HIV-1 replication than monocytes for reasons that may include the effects of different populations of miRNAs in these two cell classes. Specifically, miRs-28-3p, -150, -223, -198, and -382 exert direct or indirect negative effects on HIV-1 and are reportedly downmodulated during monocyte-to-macrophage differentiation. Here, new experimental results are presented along with reviews and analysis of published studies and publicly available datasets, supporting a broader role of miRNAs in HIV-1 restriction than would be suggested by a simple and uniform downregulation of anti-HIV miRNAs during monocyte-to-macrophage differentiation. Although miR-223 is downregulated in macrophages, other putatively antiviral miRNAs are more abundant in macrophages than in monocytes or are rare and/or variably present in both cell classes. Our analyses point to the need for further studies to determine miRNA profiles of monocytes and macrophages, including classic and newly identified subpopulations; examine the sensitivity of miRNA profiling to cell isolation and differentiation protocols; and characterize rigorously the antiviral effects of previously reported and novel predicted miRNA-HIV-1 interactions in cell-specific contexts.

Original languageEnglish (US)
Pages (from-to)1844-1864
Number of pages21
JournalViruses
Volume4
Issue number10
DOIs
StatePublished - Oct 2012

Keywords

  • Antiviral
  • HIV-1
  • Macrophage
  • Monocyte
  • Profiling
  • microRNA

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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