miRNA mutations are not a common cause of deafness

Michael S. Hildebrand, P. Dane Witmer, Shunbin Xu, Stephen S. Newton, Kimia Kahrizi, Hossein Najmabadi, David Valle, Richard J.H. Smith

Research output: Contribution to journalArticle

Abstract

Mutations in miRNA genes have been implicated in hearing loss in human families and mice. It is also possible that mutations in miRNA binding sites of inner ear targets alter gene expression levels and lead to hearing loss. To investigate these possibilities wescreened predicted target genes of the miR-183miRNAcluster knownto be expressedinthe inner ear sensory epithelium. In one Iranian family segregating autosomal recessive non-syndromic hearing loss (ARNSHL), we identified a homozygous variant in a predicted miR-96/182 binding site in the 3′UTR of the RDX (DFNB24) gene. However, in vitro functional studies showed that this site is not a functional target for miR-96/182. We extended our study to include the miR-183 genes themselves and 24 additional predicted target genes of the miRNA-183 cluster. Screening these miRNAs and target sequences in numerous families segregating either autosomal dominant non-syndromic deafness (ADNSHL) or ARNSHL did not identify any potential deafness-causing mutations. These results suggest that mutations disrupting gene regulation by the miR-183 cluster are not a common cause of human hearing loss.

Original languageEnglish (US)
Pages (from-to)646-652
Number of pages7
JournalAmerican Journal of Medical Genetics, Part A
Volume152
Issue number3
DOIs
StatePublished - Mar 1 2010

Keywords

  • ADNSHL
  • ARNSHL
  • ERM protein family
  • Radixin
  • miRNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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  • Cite this

    Hildebrand, M. S., Witmer, P. D., Xu, S., Newton, S. S., Kahrizi, K., Najmabadi, H., Valle, D., & Smith, R. J. H. (2010). miRNA mutations are not a common cause of deafness. American Journal of Medical Genetics, Part A, 152(3), 646-652. https://doi.org/10.1002/ajmg.a.33299