MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT

Yin Peng, Ying Qin, Xiaojing Zhang, Shiqi Deng, Yuan Yuan, Xianling Feng, Wangchun Chen, Fan Hu, Yuli Gao, Jieqiong He, Yulan Cheng, Yanjie Wei, Xinmin Fan, Hassan Ashktorab, Duane Smoot, Song Li, Stephen J. Meltzer, Shutong Zhuang, Na Tang, Zhe Jin

Research output: Contribution to journalArticlepeer-review

Abstract

Previous research has found that miRNA-20b is highly expressed in gastric cancer (GC), however, its function and underlying mechanism are not clear. Wnt signaling pathway, implicated in tumorigeneisis, is activated in more than 30% of GC. We would like to characterize the biological behavior of miRNA-20b in terms of modulating Wnt/β-catenin signaling and EMT. We showed that miRNA-20b inhibitors suppressed Topflash/Fopflash dependent luciferase activity and the β-catenin nuclear translocation, resulting in inhibition of Wnt pathway activity and EMT. SUFU, negatively regulating Wnt and Hedgehog signaling pathway, was proved to be targeted by miRNA-20b. Moreover, additional knockdown of SUFU alleviated the inhibitory effect on Wnt pathway activity, EMT, cell proliferation/migration and colony formation caused by miRNA-20b inhibition. In summary, miRNA-20b is an oncogenic miRNA and promoted cell proliferation, migration and EMT in GC partially by activating Wnt pathway via targeting SUFU.

Original languageEnglish (US)
Article numbere06695
JournalHeliyon
Volume7
Issue number4
DOIs
StatePublished - Apr 2021

Keywords

  • EMT
  • SUFU
  • Wnt/β-catenin
  • miRNA-20b

ASJC Scopus subject areas

  • General

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