miR-371-3 expression predicts neural differentiation propensity in human pluripotent stem cells

Hyesoo Kim, Gabsang Lee, Yosif Ganat, Eirini P. Papapetrou, Inna Lipchina, Nicholas D. Socci, Michel Sadelain, Lorenz Studer

Research output: Contribution to journalArticlepeer-review

Abstract

The use of pluripotent stem cells in regenerative medicine and disease modeling is complicated by the variation in differentiation properties between lines. In this study, we characterized 13 human embryonic stem cell (hESC) and 26 human induced pluripotent stem cell (hiPSC) lines to identify markers that predict neural differentiation behavior. At a general level, markers previously known to distinguish mouse ESCs from epiblast stem cells (EPI-SCs) correlated with neural differentiation behavior. More specifically, quantitative analysis of miR-371-3 expression prospectively identified hESC and hiPSC lines with differential neurogenic differentiation propensity and in vivo dopamine neuron engraftment potential. Transient KLF4 transduction increased miR-371-3 expression and altered neurogenic behavior and pluripotency marker expression. Conversely, suppression of miR-371-3 expression in KLF4-transduced cells rescued neural differentiation propensity. miR-371-3 expression level therefore appears to have both a predictive and a functional role in determining human pluripotent stem cell neurogenic differentiation behavior.

Original languageEnglish (US)
Pages (from-to)695-706
Number of pages12
JournalCell stem cell
Volume8
Issue number6
DOIs
StatePublished - Jun 3 2011
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

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