miR-29a suppresses growth and metastasis in papillary thyroid carcinoma by targeting AKT3

Rui Li, Jia Liu, Qun Li, Guang Chen, Xiaofang Yu

Research output: Contribution to journalArticle

Abstract

MicroRNA-29a (miR-29a) has been reported to play important roles in tumor initiation, development, and metastasis in various cancers. However, the biological function and potential mechanisms of miR-29a in papillary thyroid carcinoma (PTC) remain unclear. In the present study, we discovered that miR-29a was frequently downregulated in PTC tissues, and its expression was significantly associated with tumor size, TNM stage, and lymph node metastasis. Functional assays showed that overexpression of miR-29a markedly suppressed PTC cell proliferation, migration, and invasion and promoted PTC apoptosis and cell cycle arrest at G0/G1 phase. In vivo, miR-29a overexpression decreased tumor growth in a xenograft mouse model. Luciferase reporter assay showed that miR-29a can directly bind to the 3′ untranslated region (UTR) of AKT3 in PTC cells. Overexpreesion of miR‑29a obviously decreased AKT3 expression, thereby suppressing phosphatidylinositol 3-kinase (PI3K)/AKT pathway activation. We also confirmed that AKT3 expression was increased in PTC tissue and was inversely correlated miR-29a expression in PTC tissues. In addition, downregulation of AKT3 by siRNA mimicked the effects of miR-29a overexpression, and upregulation of AKT3 partially reversed the inhibitory effects of miR-29a. These results suggested that miR-29a could act as a tumor suppressor in PTC by targeting AKT3 and that miR-29a may potentially serve as an anti-tumor agent in the treatment of PTC.

Original languageEnglish (US)
JournalTumor Biology
DOIs
StateAccepted/In press - Oct 19 2015
Externally publishedYes

Fingerprint

MicroRNAs
Neoplasm Metastasis
Growth
Neoplasms
Papillary Thyroid cancer
Down-Regulation
Phosphatidylinositol 3-Kinase
Cell Cycle Resting Phase
G1 Phase
3' Untranslated Regions
Cell Cycle Checkpoints
Luciferases
Heterografts
Small Interfering RNA
Cell Movement
Up-Regulation
Lymph Nodes
Cell Proliferation
Apoptosis

Keywords

  • AKT3
  • miR-29a
  • Papillary thyroid carcinoma
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research

Cite this

miR-29a suppresses growth and metastasis in papillary thyroid carcinoma by targeting AKT3. / Li, Rui; Liu, Jia; Li, Qun; Chen, Guang; Yu, Xiaofang.

In: Tumor Biology, 19.10.2015.

Research output: Contribution to journalArticle

Li, Rui ; Liu, Jia ; Li, Qun ; Chen, Guang ; Yu, Xiaofang. / miR-29a suppresses growth and metastasis in papillary thyroid carcinoma by targeting AKT3. In: Tumor Biology. 2015.
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