miR-21

An androgen receptor-regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth

Judit Ribas, Xiaohua Ni, Michael Haffner, Erik A. Wentzel, Amirali Hassanzadeh Salmasi, Wasim H. Chowdhury, Tarana A. Kudrolli, S Yegnasubramanian, Jun Luo, Ron Rodriguez, Joshua T. Mendell, Shawn Lupold

Research output: Contribution to journalArticle

Abstract

Androgen receptor (AR)-mediated oncogenic pathways have not been fully elucidated. In this study, we used high-throughput microarray analysis on two AR-positive prostate cancer (CaP) cell lines to identify 16 AR-responsive microRNAs (miRNA). We focused on miR-21 because of its previously reported oncogenic activity in other cancers. We show androgen-induced AR binding to the defined miR-21 promoter, miPPR-21, suggesting direct transcriptional regulation. Inhibition of miR-21 diminished androgen-induced CaP cell proliferation, providing new evidence that miRNAs can contribute to androgen-driven cell growth. Elevated expression of miR-21 enhanced CaP tumor growth in vivo and, surprisingly, was sufficient for androgen-dependent tumors to overcome castration-mediated growth arrest. Thus, elevated miR-21 expression alone is sufficient to impart castration resistance. Moreover, quantitative reverse transcription-PCR analysis revealed elevated miR-21 expression in CaP when compared with adjacent normal tissue. These results suggest that miR-21 may contribute to CaP pathogenesis.

Original languageEnglish (US)
Pages (from-to)7165-7169
Number of pages5
JournalCancer Research
Volume69
Issue number18
DOIs
StatePublished - 2009

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Androgen Receptors
MicroRNAs
Androgens
Prostatic Neoplasms
Hormones
Castration
Growth
Neoplasms
Microarray Analysis
Reverse Transcription
Cell Proliferation
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

miR-21 : An androgen receptor-regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth. / Ribas, Judit; Ni, Xiaohua; Haffner, Michael; Wentzel, Erik A.; Salmasi, Amirali Hassanzadeh; Chowdhury, Wasim H.; Kudrolli, Tarana A.; Yegnasubramanian, S; Luo, Jun; Rodriguez, Ron; Mendell, Joshua T.; Lupold, Shawn.

In: Cancer Research, Vol. 69, No. 18, 2009, p. 7165-7169.

Research output: Contribution to journalArticle

Ribas, J, Ni, X, Haffner, M, Wentzel, EA, Salmasi, AH, Chowdhury, WH, Kudrolli, TA, Yegnasubramanian, S, Luo, J, Rodriguez, R, Mendell, JT & Lupold, S 2009, 'miR-21: An androgen receptor-regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth', Cancer Research, vol. 69, no. 18, pp. 7165-7169. https://doi.org/10.1158/0008-5472.CAN-09-1448
Ribas, Judit ; Ni, Xiaohua ; Haffner, Michael ; Wentzel, Erik A. ; Salmasi, Amirali Hassanzadeh ; Chowdhury, Wasim H. ; Kudrolli, Tarana A. ; Yegnasubramanian, S ; Luo, Jun ; Rodriguez, Ron ; Mendell, Joshua T. ; Lupold, Shawn. / miR-21 : An androgen receptor-regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth. In: Cancer Research. 2009 ; Vol. 69, No. 18. pp. 7165-7169.
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