miR-184 regulates pancreatic β-cell function according to glucose metabolism

Sudhir G. Tattikota, Thomas Rathjen, Jean Hausser, Aditya Khedkar, Uma D. Kabra, Varun Pandey, Matthias Sury, Hans Hermann Wessels, Inês G. Mollet, Lena Eliasson, Matthias Selbach, Robert P. Zinzen, Mihaela Zavolan, Sebastian Kadener, Matthias H. Tschöp, Martin Jastroch, Marc R. Friedländer, Matthew N. Poy

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


In response to fasting or hyperglycemia, the pancreatic β-cell alters its output of secreted insulin; however, the pathways governing this adaptive response are not entirely established. Although the precise role of microRNAs (miRNAs) is also unclear, a recurring theme emphasizes their function in cellular stress responses. We recently showed that miR-184, an abundant miRNA in the β-cell, regulates compensatory proliferation and secretion during insulin resistance. Consistent with previous studies showing miR-184 suppresses insulin release, expression of this miRNA was increased in islets after fasting, demonstrating an active role in the β-cell as glucose levels lower and the insulin demand ceases. Additionally, miR-184 was negatively regulated upon the administration of a sucrose-rich diet in Drosophila, demonstrating strong conservation of this pathway through evolution. Furthermore, miR-184 and its target Argonaute2 remained inversely correlated as concentrations of extracellular glucose increased, underlining a functional relationship between this miRNA and its targets. Lastly, restoration of Argonaute2 in the presence of miR-184 rescued suppression of miR-375-targeted genes, suggesting these genes act in a coordinated manner during changes in the metabolic context. Together, these results highlight the adaptive role of miR-184 according to glucose metabolism and suggest the regulatory role of this miRNA in energy homeostasis is highly conserved.

Original languageEnglish (US)
Pages (from-to)20284-20294
Number of pages11
JournalJournal of Biological Chemistry
Issue number33
StatePublished - Aug 14 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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