Minocycline inhibits LPS-induced retinal microglia activation

Ling Wang Ai, Albert C.H. Yu, Ting Lau Lok, Chong Lee, Meng Wu Le, Xiu'An Zhu, Mark O.M. Tso

Research output: Contribution to journalArticle

Abstract

Retinal neurodegenerative disease involves an inflammatory response in the retina characterized by an increase in inflammatory cytokines and activation of microglia. The degree of microglia activation may influence the extent of retinal injury following an inflammatory stimulus. Cytokines released by activated microglia regulate the influx of inflammatory cells to the damaged area. Thus, a therapeutic strategy to reduce cytokine expression in microglia would be neuroprotective. Minocycline, a semisynthetic tetracycline derivative, is known to protect rodent brain from ischemia and to inhibit microglial activation. In this study, we activated retinal microglia in culture with lipopolysaccharide (LPS) and attempted to determine whether minocycline could reduce the production of cytokines from activated microglia at both gene and protein levels. Changes in inflammatory cytokines, TNF-alpha and IL-1beta, were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) in the presence or absence of LPS. We also measured the levels of nitric oxide (NO) by the nitrate reductase method under similar conditions. LPS treatment induced a significant upregulation of the mRNA and release of TNF-alpha, IL-1beta, and NO from retinal microglia. Minocycline inhibited these releases. Thus, minocycline might exert its antiinflammatory effect on microglia by inhibiting the expression and release of TNF-alpha, IL-1beta, and NO.

Original languageEnglish (US)
Pages (from-to)152-158
Number of pages7
JournalNeurochemistry International
Volume47
Issue number1-2 SPEC. ISS.
DOIs
StatePublished - Jul 1 2005

Keywords

  • IL-1beta
  • LPS
  • Microglia
  • Minocycline
  • NO
  • Neurodegenerative disease
  • Retina
  • TNF-alpha

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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  • Cite this

    Ai, L. W., Yu, A. C. H., Lok, T. L., Lee, C., Le, M. W., Zhu, XA., & Tso, M. O. M. (2005). Minocycline inhibits LPS-induced retinal microglia activation. Neurochemistry International, 47(1-2 SPEC. ISS.), 152-158. https://doi.org/10.1016/j.neuint.2005.04.018