Abstract
To identify novel, tumor-specific target antigens for vaccine development, we studied immune responses to P. polypeptide, an Mr 110,000 integral melanosomal membrane protein associated with the Prader-Willi syndrome. Together with expressed sequence tag (EST) and serial analyses of gene expression (SAGE) library analyses, reverse transcription-PCR and Northern blotting verified that P. polypeptide expression was limited to melanoma and melanocytes. A single dominant epitope corresponding to positions 427-435 (IMLCLIAAV) was identified using allele-specific epitope forecasting combined with work in HLA-A*0201/Kb transgenic mice. This epitope was then used to generate de novo human P. polypeptide-specific CD8+ T cells capable of recognizing P. polypeptide expressing human tumor cell lines in an HLA-A*0201-restricted fashion. Thus, P. polypeptide may be valuable in the creation of novel therapeutic anticancer vaccines.
Original language | English (US) |
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Pages (from-to) | 8100-8104 |
Number of pages | 5 |
Journal | Cancer Research |
Volume | 61 |
Issue number | 22 |
State | Published - Nov 15 2001 |
ASJC Scopus subject areas
- Oncology
- Cancer Research