Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Uri S. Markakpo, Kwabena M. Bosompem, Mawuli Dzodzomenyo, Anthony Danso-Appiah, Edward E. Essuman, William K. Anyan, Mitsuko Suzuki, Judith K. Stephens, Isaac Anim-Baidoo, Richard H. Asmah, Michael F. Ofori, Parnor Madjitey, Jonas B. Danquah, Naa Adjeley Frempong, Kofi D. Kwofie, Michael Amoa-Bosompem, David Sullivan, Julius N. Fobil, Isabella A. Quakyi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

Original languageEnglish (US)
Pages (from-to)1263-1271
Number of pages9
JournalTropical Medicine and International Health
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2016

Keywords

  • Plasmodium
  • dipstick antigen monoclonal antibody
  • malaria

ASJC Scopus subject areas

  • Parasitology
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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