Minimally-invasive technique for injection into rat optic nerve

Kateryna Raykova, Melina Jones, Hwa Huang, Paul F. Hoffman, Michael Levy

Research output: Contribution to journalArticle

Abstract

The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

Original languageEnglish (US)
Article numbere52249
JournalJournal of Visualized Experiments
Volume2015
Issue number99
DOIs
StatePublished - May 19 2015

Fingerprint

Optic Nerve
Rats
Optics
Injections
Direct injection
Stem Cell Research
Dissection
Minimally Invasive Surgical Procedures
Traction
Neurology
Stem cells
Surgery
Sutures
Regeneration
Bone
Visualization
Central Nervous System
Bone and Bones

Keywords

  • Injection
  • Issue 99
  • Neuroscience
  • Optic nerve
  • Optic neuritis
  • Optic neuropathy
  • Regeneration
  • Stem cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Minimally-invasive technique for injection into rat optic nerve. / Raykova, Kateryna; Jones, Melina; Huang, Hwa; Hoffman, Paul F.; Levy, Michael.

In: Journal of Visualized Experiments, Vol. 2015, No. 99, e52249, 19.05.2015.

Research output: Contribution to journalArticle

Raykova, Kateryna ; Jones, Melina ; Huang, Hwa ; Hoffman, Paul F. ; Levy, Michael. / Minimally-invasive technique for injection into rat optic nerve. In: Journal of Visualized Experiments. 2015 ; Vol. 2015, No. 99.
@article{ef0f75410d9b4cbf91010f5452f3a454,
title = "Minimally-invasive technique for injection into rat optic nerve",
abstract = "The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.",
keywords = "Injection, Issue 99, Neuroscience, Optic nerve, Optic neuritis, Optic neuropathy, Regeneration, Stem cells",
author = "Kateryna Raykova and Melina Jones and Hwa Huang and Hoffman, {Paul F.} and Michael Levy",
year = "2015",
month = "5",
day = "19",
doi = "10.3791/52249",
language = "English (US)",
volume = "2015",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "99",

}

TY - JOUR

T1 - Minimally-invasive technique for injection into rat optic nerve

AU - Raykova, Kateryna

AU - Jones, Melina

AU - Huang, Hwa

AU - Hoffman, Paul F.

AU - Levy, Michael

PY - 2015/5/19

Y1 - 2015/5/19

N2 - The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

AB - The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

KW - Injection

KW - Issue 99

KW - Neuroscience

KW - Optic nerve

KW - Optic neuritis

KW - Optic neuropathy

KW - Regeneration

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=84941254933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941254933&partnerID=8YFLogxK

U2 - 10.3791/52249

DO - 10.3791/52249

M3 - Article

C2 - 26068024

AN - SCOPUS:84941254933

VL - 2015

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 99

M1 - e52249

ER -