Minced urothelium to create epithelialized subcutaneous conduits

Magdalena Fossum, Baraa Zuhaili, Juri Bergmann, Malte Spielmann, Tobias Hirsch, Elof Eriksson

Research output: Contribution to journalArticle

Abstract

Purpose: We used in vivo cell expansion to create 3-dimensional subcutaneous conduits lined with an inner layer of autologous urothelial mucosa. Materials and Methods: Laparotomy and excision of a fifth of the bladder were done in 5 female Yorkshire pigs (Parsons Farm, Westhampton, Massachusetts) under general anesthesia. After mechanical removal of the detrusor muscle the bladder mucosa was minced to obtain 0.2 × 0.8 × 0.8 mm particles, which were attached to the outer surface of latex tubes using a thin layer of fibrin glue. Seven to 10 tubes were placed in the abdominal wall subcutaneous tissue in each original donor pig with tubes lacking particles serving as controls. Biopsy was done 1 to 4 weeks after transplantation for histological evaluation. Results: One week after transplantation particles were still present in the granulation tissue. At 2 weeks the epithelium was differentiated with transitional uroepithelium facing the lumen, ie toward the tube. No epithelium was detected around control tubes. Conclusions: After autologous transplantation of bladder mucosal particles organized in 3-dimensional fashion in pig subcutaneous tissue the transplanted cells proliferated, migrated and reorganized to form a continuous epithelial lining facing the lumen. This novel approach to urothelial transplantation may allow successful formation of a conduit to the bladder or of a neourethra.

Original languageEnglish (US)
Pages (from-to)757-761
Number of pages5
JournalJournal of Urology
Volume184
Issue number2
DOIs
StatePublished - Jun 21 2010
Externally publishedYes

Keywords

  • epithelium
  • swine
  • tissue engineering
  • urinary bladder
  • urothelium

ASJC Scopus subject areas

  • Urology

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  • Cite this

    Fossum, M., Zuhaili, B., Bergmann, J., Spielmann, M., Hirsch, T., & Eriksson, E. (2010). Minced urothelium to create epithelialized subcutaneous conduits. Journal of Urology, 184(2), 757-761. https://doi.org/10.1016/j.juro.2010.04.031