MiMIC: A highly versatile transposon insertion resource for engineering Drosophila melanogaster genes

Koen J T Venken, Karen L. Schulze, Nele A. Haelterman, Hongling Pan, Yuchun He, Martha Evans-Holm, Joseph W. Carlson, Robert W. Levis, Allan C. Spradling, Roger A. Hoskins, Hugo J. Bellen

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow + marker flanked by two inverted bacteriophage Î ■C31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNA manipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.

Original languageEnglish (US)
Pages (from-to)737-747
Number of pages11
JournalNature Methods
Volume8
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

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