Milton controls the early acquisition of mitochondria by Drosophila oocytes

Rachel T. Cox, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Mitochondria in many species enter the young oocyte en mass from interconnected germ cells to generate the large aggregate known as the Balbiani body. Organelles and germ plasm components frequently associate with this structure. Balbiani body mitochondria are thought to populate the germ line, ensuring that their genomes will be inherited preferentially. We find that milton, a gene whose product was previously shown to associate with Kinesin and to mediate axonal transport of mitochondria, is needed to form a normal Balbiani body. In addition, germ cells mutant for some milton or Kinesin heavy chain (Khc) alleles transport mitochondria to the oocyte prematurely and excessively, without disturbing Balbiani body-associated components. Our observations show that the oocyte acquires the majority of its mitochondria by competitive bidirectional transport along microtubules mediated by the Milton adaptor. These experiments provide a molecular explanation for Balbiani body formation and, surprisingly, show that viable fertile offspring can be obtained from eggs in which the normal program of mitochondrial acquisition has been severely perturbed.

Original languageEnglish (US)
Pages (from-to)3371-3377
Number of pages7
JournalDevelopment
Volume133
Issue number17
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Drosophila
Oocytes
Mitochondria
Germ Cells
Kinesin
Axonal Transport
Microtubules
Organelles
Eggs
Alleles
Genome
Genes

Keywords

  • Balbiani body
  • Dynein
  • Fusome
  • Kinesin
  • Microtubule
  • Mitochondria
  • Oogenesis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Milton controls the early acquisition of mitochondria by Drosophila oocytes. / Cox, Rachel T.; Spradling, Allan C.

In: Development, Vol. 133, No. 17, 09.2006, p. 3371-3377.

Research output: Contribution to journalArticle

Cox, Rachel T. ; Spradling, Allan C. / Milton controls the early acquisition of mitochondria by Drosophila oocytes. In: Development. 2006 ; Vol. 133, No. 17. pp. 3371-3377.
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