Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes: Depletion and reaccumulation

Peter Bergsten, Gabriel Amitai, John Kehrl, Kuldeep R. Dhariwal, Harvey G. Klein, Mark Levine

Research output: Contribution to journalArticlepeer-review

Abstract

Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components in millimolar concentration. Intracellular ascorbic acid was depleted >96% during cell culture and was rapidly reaccumulated after addition of physiologic concentrations of ascorbic acid to the extracellular medium. Purified cells maintained concentration gradients of ascorbic acid as large as 100-fold across the plasma membrane. The ability to vary intracellular ascorbic acid concentrations over such a wide range makes it possible for the first time in these cells to study ascorbic acid function in direct relationship to intracellular concentration.

Original languageEnglish (US)
Pages (from-to)2584-2587
Number of pages4
JournalJournal of Biological Chemistry
Volume265
Issue number5
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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