MIF

A lymphokine in lens

Noriko Esumi, Debasish Sinha, Vishwas Paralkar, Frances Noonan, Graciana Diez Rox, Richard Lang, Graeme Wistow

Research output: Contribution to journalArticle

Abstract

Purpose. To examine the mechanisms of expression and functionality of macrophage migration inhibitory factor (MIF), a lymphokine and delayed early response gene expressed in equatorial cells of the lens. Methods. The genes for human and mouse MBF have been cloned and sequenced. Promoter deletions were assayed by reporter gene expression in cell culture. MIF induction by growth factors was analyzed by northern blot and by reporter gene assay. Recombinant mouse MIF was expressed using the pET system. MIF activity in lens extracts was assayed by standard migration inhibition assay. Mouse MIF sense and antisense expression vectors are being used to modify levels of MIF in cultured cells and transgenic mice. Results and Conclusions. MIF is a delayed early response gene induced by different families of growth factors and is therefore a candidate for involvement in the cascade of events from growth factor reception to lens cell differentiation. The promoter of the human MIF gene has been defined by functional analysis. The expression of MIF in the encapsulated lens and other immunologically privileged sites suggests that it has roles distinct from that of a lymphokine. Even so, the presence of MIF in lens raises the possibility that this lymphokine activity could play a part in lens-induced ocular inflammations.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Lymphokines
Lenses
Intercellular Signaling Peptides and Proteins
Reporter Genes
Genes
Human Migration
Macrophage Migration-Inhibitory Factors
Northern Blotting
Transgenic Mice
Cell Differentiation
Cultured Cells
Cell Culture Techniques
Inflammation
Gene Expression

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Esumi, N., Sinha, D., Paralkar, V., Noonan, F., Rox, G. D., Lang, R., & Wistow, G. (1996). MIF: A lymphokine in lens. Investigative Ophthalmology and Visual Science, 37(3).

MIF : A lymphokine in lens. / Esumi, Noriko; Sinha, Debasish; Paralkar, Vishwas; Noonan, Frances; Rox, Graciana Diez; Lang, Richard; Wistow, Graeme.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Esumi, N, Sinha, D, Paralkar, V, Noonan, F, Rox, GD, Lang, R & Wistow, G 1996, 'MIF: A lymphokine in lens', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Esumi N, Sinha D, Paralkar V, Noonan F, Rox GD, Lang R et al. MIF: A lymphokine in lens. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Esumi, Noriko ; Sinha, Debasish ; Paralkar, Vishwas ; Noonan, Frances ; Rox, Graciana Diez ; Lang, Richard ; Wistow, Graeme. / MIF : A lymphokine in lens. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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