Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment

Yisel M. Cantres-Rosario, Frances M. Acevedo-Mariani, Juliana Pérez-Laspiur, William E. Haskins, Marines Plaud, Yadira M. Cantres-Rosario, Richard Skolasky, Israel Méndez-Bermúdez, Valerie Wojna, Loyda M. Meléndez

Research output: Contribution to journalArticle

Abstract

Objective: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retrovi-ral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. Methods: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. Results: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. Conclusions: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI.

Original languageEnglish (US)
Article numbere0181779
JournalPLoS One
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2017

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Macrophages
Microwaves
Proteomics
proteomics
macrophages
HIV
Proteins
proteins
monocytes
cognition
therapeutics
Cognition
Phosphogluconate Dehydrogenase
phosphogluconate dehydrogenase
blood-brain barrier
neurotoxins
Cognitive Dysfunction
Liquid chromatography
Neurotoxins
Neurology

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cantres-Rosario, Y. M., Acevedo-Mariani, F. M., Pérez-Laspiur, J., Haskins, W. E., Plaud, M., Cantres-Rosario, Y. M., ... Meléndez, L. M. (2017). Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. PLoS One, 12(7), [e0181779]. https://doi.org/10.1371/journal.pone.0181779

Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. / Cantres-Rosario, Yisel M.; Acevedo-Mariani, Frances M.; Pérez-Laspiur, Juliana; Haskins, William E.; Plaud, Marines; Cantres-Rosario, Yadira M.; Skolasky, Richard; Méndez-Bermúdez, Israel; Wojna, Valerie; Meléndez, Loyda M.

In: PLoS One, Vol. 12, No. 7, e0181779, 01.07.2017.

Research output: Contribution to journalArticle

Cantres-Rosario, YM, Acevedo-Mariani, FM, Pérez-Laspiur, J, Haskins, WE, Plaud, M, Cantres-Rosario, YM, Skolasky, R, Méndez-Bermúdez, I, Wojna, V & Meléndez, LM 2017, 'Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment', PLoS One, vol. 12, no. 7, e0181779. https://doi.org/10.1371/journal.pone.0181779
Cantres-Rosario YM, Acevedo-Mariani FM, Pérez-Laspiur J, Haskins WE, Plaud M, Cantres-Rosario YM et al. Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. PLoS One. 2017 Jul 1;12(7). e0181779. https://doi.org/10.1371/journal.pone.0181779
Cantres-Rosario, Yisel M. ; Acevedo-Mariani, Frances M. ; Pérez-Laspiur, Juliana ; Haskins, William E. ; Plaud, Marines ; Cantres-Rosario, Yadira M. ; Skolasky, Richard ; Méndez-Bermúdez, Israel ; Wojna, Valerie ; Meléndez, Loyda M. / Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. In: PLoS One. 2017 ; Vol. 12, No. 7.
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abstract = "Objective: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retrovi-ral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. Methods: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. Results: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. Conclusions: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI.",
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AU - Plaud, Marines

AU - Cantres-Rosario, Yadira M.

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N2 - Objective: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retrovi-ral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. Methods: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. Results: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. Conclusions: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI.

AB - Objective: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retrovi-ral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy. Methods: MDM were isolated from the peripheral blood of 14 HIV-seropositive women characterized for neurocognitive function, including: four normal cognition (NC), five asymptomatic (A), and five with cognitive impaired (CI). Proteins from macrophage lysates were isobaric-labeled with the microwave and magnetic (M2) sample preparation method followed by liquid chromatography-tandem mass spectrometry-based protein identification and quantification. Differences in protein abundance across groups classified by HACI status were determined using analysis of variance. Results: A total of 2,519 proteins were identified with 2 or more peptides and 28 proteins were quantified as differentially expressed. Statistical analysis revealed increased abundance of 17 proteins in patients with HACI (p<0.05), including several enzymes associated to the glucose metabolism. Western blot confirmed increased expression of 6-Phosphogluconate dehydrogenase and L-Plastin in A and CI patients over NC and HIV seronegatives. Conclusions: This is the first quantitative proteomics study exploring the changes in protein abundance of macrophages isolated from patients with HACI. Further studies are warranted to determine if these proteins may be target candidates for therapy development against HACI.

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