Microtubule-nucleus interactions in dictyostelium discoideum mediated by central motor kinesins

Irina Tikhonenko, Dilip K. Nag, Douglas Robinson, Michael P. Koonce

Research output: Contribution to journalArticle

Abstract

Kinesins are a diverse superfamily of motor proteins that drive organelles and other microtubule-based movements in eukaryotic cells. These motors play important roles in multiple events during both interphase and cell division. Dictyostelium discoideum contains 13 kinesin motors, 12 of which are grouped into nine families, plus one orphan. Functions for 11 of the 13 motors have been previously investigated; we address here the activities of the two remaining kinesins, both isoforms with central motor domains. Kif6 (of the kinesin-13 family) appears to be essential for cell viability. The partial knockdown of Kif6 with RNA interference generates mitotic defects (lagging chromosomes and aberrant spindle assemblies} that are consistent with kinesin-13 disruptions in other organisms. However, the orphan motor Kif9 participates in a completely novel kinesin activity, one that maintains a connection between the microtubule-organizing center (MTOC) and nucleus during interphase. kif9 null cell growth is impaired, and the MTOC appears to disconnect from its normally tight nuclear linkage. Mitotic spindles elongate in a normal fashion in kif9δ cells, but we hypothesize that this kinesin is important for positioning the MTOC into the nuclear envelope during prophase. This function would be significant for the early steps of cell division and also may play a role in regulating centrosome replication.

Original languageEnglish (US)
Pages (from-to)723-731
Number of pages9
JournalEukaryotic Cell
Volume8
Issue number5
DOIs
StatePublished - May 2009

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Kinesin
Dictyostelium
Microtubules
Microtubule-Organizing Center
Interphase
Cell Division
Prophase
Null Lymphocytes
Centrosome
Spindle Apparatus
Nuclear Envelope
Eukaryotic Cells
RNA Interference
Organelles
Cell Survival
Protein Isoforms
Chromosomes
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Microtubule-nucleus interactions in dictyostelium discoideum mediated by central motor kinesins. / Tikhonenko, Irina; Nag, Dilip K.; Robinson, Douglas; Koonce, Michael P.

In: Eukaryotic Cell, Vol. 8, No. 5, 05.2009, p. 723-731.

Research output: Contribution to journalArticle

Tikhonenko, Irina ; Nag, Dilip K. ; Robinson, Douglas ; Koonce, Michael P. / Microtubule-nucleus interactions in dictyostelium discoideum mediated by central motor kinesins. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 5. pp. 723-731.
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