Microtubule-associated protein 2 (MAP-2): a sensitive marker of seizure-related brain damage

G. P.H. Ballough; L. J. Martin; F. J. Cann; J. S. Graham; C. D. Smith; C. E. Kling; J. S. Forster; S. Phann; M. G. Filbert

doi:10.1016/0165-0270(95)00019-Q

Microtubule-associated protein 2 (MAP-2): a sensitive marker of seizure-related brain damage

G. P.H. Ballough, L. J. Martin, F. J. Cann, J. S. Graham, C. D. Smith, C. E. Kling, J. S. Forster, S. Phann, M. G. Filbert

School of Medicine

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

We have assessed the efficacy of MAP-2 immunohistochemistry as a marker of seizure-related brain damage and its suitability for quantitation of the damage using densitometric and morphometric image analysis. Seizures were produced in rats by administration of 1.5 LD₅₀ soman, an irreversible AChE inhibitor. Our results demonstrate that neuronal damage, assessed using hematoxylin and eosin, and cresyl violet staining, was colocalized on adjacent serial sections with clearly demarcated reductions in MAP-2 staining. The most severely damaged brain regions were devoid of MAP-2 staining. Reductions in MAP-2 immunostaining were found to be exceptionally well suited for quantitation using densitometric and morphometric image analysis. This study represents the first demonstration of seizure-induced excitotoxic alterations in MAP-2.

Original language	English (US)
Pages (from-to)	23-32
Number of pages	10
Journal	Journal of Neuroscience Methods
Volume	61
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-0270(95)00019-Q
State	Published - 1995

Keywords

Cresyl Violet
Eosin
GFAP
Hematoxylin
Microtubule-associated protein-2
Neurotoxicity
Seizure
Soman

ASJC Scopus subject areas

General Neuroscience

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10.1016/0165-0270(95)00019-Q

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title = "Microtubule-associated protein 2 (MAP-2): a sensitive marker of seizure-related brain damage",

abstract = "We have assessed the efficacy of MAP-2 immunohistochemistry as a marker of seizure-related brain damage and its suitability for quantitation of the damage using densitometric and morphometric image analysis. Seizures were produced in rats by administration of 1.5 LD50 soman, an irreversible AChE inhibitor. Our results demonstrate that neuronal damage, assessed using hematoxylin and eosin, and cresyl violet staining, was colocalized on adjacent serial sections with clearly demarcated reductions in MAP-2 staining. The most severely damaged brain regions were devoid of MAP-2 staining. Reductions in MAP-2 immunostaining were found to be exceptionally well suited for quantitation using densitometric and morphometric image analysis. This study represents the first demonstration of seizure-induced excitotoxic alterations in MAP-2.",

keywords = "Cresyl Violet, Eosin, GFAP, Hematoxylin, Microtubule-associated protein-2, Neurotoxicity, Seizure, Soman",

author = "Ballough, {G. P.H.} and Martin, {L. J.} and Cann, {F. J.} and Graham, {J. S.} and Smith, {C. D.} and Kling, {C. E.} and Forster, {J. S.} and S. Phann and Filbert, {M. G.}",

year = "1995",

doi = "10.1016/0165-0270(95)00019-Q",

language = "English (US)",

volume = "61",

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T1 - Microtubule-associated protein 2 (MAP-2)

T2 - a sensitive marker of seizure-related brain damage

AU - Ballough, G. P.H.

AU - Martin, L. J.

AU - Cann, F. J.

AU - Graham, J. S.

AU - Smith, C. D.

AU - Kling, C. E.

AU - Forster, J. S.

AU - Phann, S.

AU - Filbert, M. G.

PY - 1995

Y1 - 1995

N2 - We have assessed the efficacy of MAP-2 immunohistochemistry as a marker of seizure-related brain damage and its suitability for quantitation of the damage using densitometric and morphometric image analysis. Seizures were produced in rats by administration of 1.5 LD50 soman, an irreversible AChE inhibitor. Our results demonstrate that neuronal damage, assessed using hematoxylin and eosin, and cresyl violet staining, was colocalized on adjacent serial sections with clearly demarcated reductions in MAP-2 staining. The most severely damaged brain regions were devoid of MAP-2 staining. Reductions in MAP-2 immunostaining were found to be exceptionally well suited for quantitation using densitometric and morphometric image analysis. This study represents the first demonstration of seizure-induced excitotoxic alterations in MAP-2.

AB - We have assessed the efficacy of MAP-2 immunohistochemistry as a marker of seizure-related brain damage and its suitability for quantitation of the damage using densitometric and morphometric image analysis. Seizures were produced in rats by administration of 1.5 LD50 soman, an irreversible AChE inhibitor. Our results demonstrate that neuronal damage, assessed using hematoxylin and eosin, and cresyl violet staining, was colocalized on adjacent serial sections with clearly demarcated reductions in MAP-2 staining. The most severely damaged brain regions were devoid of MAP-2 staining. Reductions in MAP-2 immunostaining were found to be exceptionally well suited for quantitation using densitometric and morphometric image analysis. This study represents the first demonstration of seizure-induced excitotoxic alterations in MAP-2.

KW - Cresyl Violet

KW - Eosin

KW - GFAP

KW - Hematoxylin

KW - Microtubule-associated protein-2

KW - Neurotoxicity

KW - Seizure

KW - Soman

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AN - SCOPUS:0028828425

SN - 0165-0270

VL - 61

SP - 23

EP - 32

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 1-2

ER -

Microtubule-associated protein 2 (MAP-2): a sensitive marker of seizure-related brain damage

Abstract

Keywords

ASJC Scopus subject areas

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