Microsurgical rat varicocele model

Bobby B. Najari, Philip S. Li, Ranjith Ramasamy, Matthew Katz, Seema Sheth, Brian Robinson, Haolin Chen, Barry R Zirkin, Peter N. Schlegel, Marc Goldstein

Research output: Contribution to journalArticle

Abstract

Purpose A rat varicocele model using partial occlusion of the left renal vein was described previously. Reproducibility in creating this model has met with varied success. Alternate routes of testicular venous drainage may negate the effect of partial renal vein occlusion on varicocele creation. We hypothesized that varicocele induction would be more effective if microsurgical ligation of the gonadal venous drainage to the common iliac vein was combined with partial occlusion of the left renal vein. Materials and Methods We randomly assigned a total of 36 rats to 3 groups, including sham surgery, partial renal vein occlusion alone (the classic technique) and microsurgical ligation. Half of the rats in each group were evaluated at 5 and 12 weeks, respectively. We evaluated internal gonadal vein and spermatic cord diameter, testicular weight, cauda epididymal sperm concentration and motility and testicular histology using the Johnsen score as well as serum and intratesticular testosterone and dihydrotestosterone. Results Five weeks after varicocele creation the microsurgical ligation group had a larger spermatic cord diameter and lower Johnsen scores than rats in the classic technique and sham surgery groups. At 12 weeks the microsurgical ligation group had a larger spermatic cord diameter, lower cauda epididymal sperm concentration, lower sperm motility and worse histology than the classic technique and sham surgery groups. There was no difference in serum androgen outcomes but the microsurgical ligation group had lower intratesticular androgens. Conclusions Adding microsurgical ligation of testicular vein collaterals in the pelvis to partial renal vein occlusion appears to improve the effectiveness of creating a rat varicocele model.

Original languageEnglish (US)
Pages (from-to)548-553
Number of pages6
JournalJournal of Urology
Volume191
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Varicocele
Renal Veins
Ligation
Spermatic Cord
Sperm Motility
Androgens
Drainage
Veins
Histology
Iliac Vein
Dihydrotestosterone
Serum
Pelvis
Testosterone
Spermatozoa
Weights and Measures

Keywords

  • microsurgery
  • rats
  • spermatic cord
  • testis
  • varicocele

ASJC Scopus subject areas

  • Urology

Cite this

Najari, B. B., Li, P. S., Ramasamy, R., Katz, M., Sheth, S., Robinson, B., ... Goldstein, M. (2014). Microsurgical rat varicocele model. Journal of Urology, 191(2), 548-553. https://doi.org/10.1016/j.juro.2013.08.011

Microsurgical rat varicocele model. / Najari, Bobby B.; Li, Philip S.; Ramasamy, Ranjith; Katz, Matthew; Sheth, Seema; Robinson, Brian; Chen, Haolin; Zirkin, Barry R; Schlegel, Peter N.; Goldstein, Marc.

In: Journal of Urology, Vol. 191, No. 2, 02.2014, p. 548-553.

Research output: Contribution to journalArticle

Najari, BB, Li, PS, Ramasamy, R, Katz, M, Sheth, S, Robinson, B, Chen, H, Zirkin, BR, Schlegel, PN & Goldstein, M 2014, 'Microsurgical rat varicocele model', Journal of Urology, vol. 191, no. 2, pp. 548-553. https://doi.org/10.1016/j.juro.2013.08.011
Najari BB, Li PS, Ramasamy R, Katz M, Sheth S, Robinson B et al. Microsurgical rat varicocele model. Journal of Urology. 2014 Feb;191(2):548-553. https://doi.org/10.1016/j.juro.2013.08.011
Najari, Bobby B. ; Li, Philip S. ; Ramasamy, Ranjith ; Katz, Matthew ; Sheth, Seema ; Robinson, Brian ; Chen, Haolin ; Zirkin, Barry R ; Schlegel, Peter N. ; Goldstein, Marc. / Microsurgical rat varicocele model. In: Journal of Urology. 2014 ; Vol. 191, No. 2. pp. 548-553.
@article{a4e2af681e7b4fcab71e52735f9ba480,
title = "Microsurgical rat varicocele model",
abstract = "Purpose A rat varicocele model using partial occlusion of the left renal vein was described previously. Reproducibility in creating this model has met with varied success. Alternate routes of testicular venous drainage may negate the effect of partial renal vein occlusion on varicocele creation. We hypothesized that varicocele induction would be more effective if microsurgical ligation of the gonadal venous drainage to the common iliac vein was combined with partial occlusion of the left renal vein. Materials and Methods We randomly assigned a total of 36 rats to 3 groups, including sham surgery, partial renal vein occlusion alone (the classic technique) and microsurgical ligation. Half of the rats in each group were evaluated at 5 and 12 weeks, respectively. We evaluated internal gonadal vein and spermatic cord diameter, testicular weight, cauda epididymal sperm concentration and motility and testicular histology using the Johnsen score as well as serum and intratesticular testosterone and dihydrotestosterone. Results Five weeks after varicocele creation the microsurgical ligation group had a larger spermatic cord diameter and lower Johnsen scores than rats in the classic technique and sham surgery groups. At 12 weeks the microsurgical ligation group had a larger spermatic cord diameter, lower cauda epididymal sperm concentration, lower sperm motility and worse histology than the classic technique and sham surgery groups. There was no difference in serum androgen outcomes but the microsurgical ligation group had lower intratesticular androgens. Conclusions Adding microsurgical ligation of testicular vein collaterals in the pelvis to partial renal vein occlusion appears to improve the effectiveness of creating a rat varicocele model.",
keywords = "microsurgery, rats, spermatic cord, testis, varicocele",
author = "Najari, {Bobby B.} and Li, {Philip S.} and Ranjith Ramasamy and Matthew Katz and Seema Sheth and Brian Robinson and Haolin Chen and Zirkin, {Barry R} and Schlegel, {Peter N.} and Marc Goldstein",
year = "2014",
month = "2",
doi = "10.1016/j.juro.2013.08.011",
language = "English (US)",
volume = "191",
pages = "548--553",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Microsurgical rat varicocele model

AU - Najari, Bobby B.

AU - Li, Philip S.

AU - Ramasamy, Ranjith

AU - Katz, Matthew

AU - Sheth, Seema

AU - Robinson, Brian

AU - Chen, Haolin

AU - Zirkin, Barry R

AU - Schlegel, Peter N.

AU - Goldstein, Marc

PY - 2014/2

Y1 - 2014/2

N2 - Purpose A rat varicocele model using partial occlusion of the left renal vein was described previously. Reproducibility in creating this model has met with varied success. Alternate routes of testicular venous drainage may negate the effect of partial renal vein occlusion on varicocele creation. We hypothesized that varicocele induction would be more effective if microsurgical ligation of the gonadal venous drainage to the common iliac vein was combined with partial occlusion of the left renal vein. Materials and Methods We randomly assigned a total of 36 rats to 3 groups, including sham surgery, partial renal vein occlusion alone (the classic technique) and microsurgical ligation. Half of the rats in each group were evaluated at 5 and 12 weeks, respectively. We evaluated internal gonadal vein and spermatic cord diameter, testicular weight, cauda epididymal sperm concentration and motility and testicular histology using the Johnsen score as well as serum and intratesticular testosterone and dihydrotestosterone. Results Five weeks after varicocele creation the microsurgical ligation group had a larger spermatic cord diameter and lower Johnsen scores than rats in the classic technique and sham surgery groups. At 12 weeks the microsurgical ligation group had a larger spermatic cord diameter, lower cauda epididymal sperm concentration, lower sperm motility and worse histology than the classic technique and sham surgery groups. There was no difference in serum androgen outcomes but the microsurgical ligation group had lower intratesticular androgens. Conclusions Adding microsurgical ligation of testicular vein collaterals in the pelvis to partial renal vein occlusion appears to improve the effectiveness of creating a rat varicocele model.

AB - Purpose A rat varicocele model using partial occlusion of the left renal vein was described previously. Reproducibility in creating this model has met with varied success. Alternate routes of testicular venous drainage may negate the effect of partial renal vein occlusion on varicocele creation. We hypothesized that varicocele induction would be more effective if microsurgical ligation of the gonadal venous drainage to the common iliac vein was combined with partial occlusion of the left renal vein. Materials and Methods We randomly assigned a total of 36 rats to 3 groups, including sham surgery, partial renal vein occlusion alone (the classic technique) and microsurgical ligation. Half of the rats in each group were evaluated at 5 and 12 weeks, respectively. We evaluated internal gonadal vein and spermatic cord diameter, testicular weight, cauda epididymal sperm concentration and motility and testicular histology using the Johnsen score as well as serum and intratesticular testosterone and dihydrotestosterone. Results Five weeks after varicocele creation the microsurgical ligation group had a larger spermatic cord diameter and lower Johnsen scores than rats in the classic technique and sham surgery groups. At 12 weeks the microsurgical ligation group had a larger spermatic cord diameter, lower cauda epididymal sperm concentration, lower sperm motility and worse histology than the classic technique and sham surgery groups. There was no difference in serum androgen outcomes but the microsurgical ligation group had lower intratesticular androgens. Conclusions Adding microsurgical ligation of testicular vein collaterals in the pelvis to partial renal vein occlusion appears to improve the effectiveness of creating a rat varicocele model.

KW - microsurgery

KW - rats

KW - spermatic cord

KW - testis

KW - varicocele

UR - http://www.scopus.com/inward/record.url?scp=84891923000&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891923000&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2013.08.011

DO - 10.1016/j.juro.2013.08.011

M3 - Article

C2 - 23954374

AN - SCOPUS:84891923000

VL - 191

SP - 548

EP - 553

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 2

ER -