Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery

Xiangying Du, Yuesong Yang, Catherine Le Visage, Hunter H. Chen, Robert De Jong, Bensheng Qiu, Danming Wang, Kam W. Leong, Ulrike Maria Hamper, Xiaoming Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study was to test the feasibility of using a novel biodegradable microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. We first encapsulated green fluoresent protein (GFP) gene into the microspheres and validated the echogenic property of GFP-plasmid/microspheres with intramuscular injection. Then, the GFP-plasmid/microspheres were transferred into the arteries of pigs via a catheter-based delivery procedure under ultrasound imaging. Strong echogenic signal reflected from the microspheres indicated the destination of genes delivered, whereas functional gene expression was confirmed by immunohistochemical examination. Microspheres can be feasible contrast agents/gene vectors in ultrasound imaging-based vascular gene delivery in vivo.

Original languageEnglish (US)
Title of host publicationProceedings - International Symposium on Biomedical Imaging
PublisherIEEE Computer Society
Pages989-992
Number of pages4
Volume2002-January
ISBN (Print)078037584X
DOIs
StatePublished - 2002
EventIEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States
Duration: Jul 7 2002Jul 10 2002

Other

OtherIEEE International Symposium on Biomedical Imaging, ISBI 2002
CountryUnited States
CityWashington
Period7/7/027/10/02

Fingerprint

Microspheres
Contrast Media
Blood Vessels
Ultrasonography
Genes
Ultrasonics
Imaging techniques
Proteins
Plasmids
Catheters
Intramuscular Injections
Gene expression
Swine
Arteries
Gene Expression

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Du, X., Yang, Y., Le Visage, C., Chen, H. H., De Jong, R., Qiu, B., ... Yang, X. (2002). Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. In Proceedings - International Symposium on Biomedical Imaging (Vol. 2002-January, pp. 989-992). [1029429] IEEE Computer Society. https://doi.org/10.1109/ISBI.2002.1029429

Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. / Du, Xiangying; Yang, Yuesong; Le Visage, Catherine; Chen, Hunter H.; De Jong, Robert; Qiu, Bensheng; Wang, Danming; Leong, Kam W.; Hamper, Ulrike Maria; Yang, Xiaoming.

Proceedings - International Symposium on Biomedical Imaging. Vol. 2002-January IEEE Computer Society, 2002. p. 989-992 1029429.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Du, X, Yang, Y, Le Visage, C, Chen, HH, De Jong, R, Qiu, B, Wang, D, Leong, KW, Hamper, UM & Yang, X 2002, Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. in Proceedings - International Symposium on Biomedical Imaging. vol. 2002-January, 1029429, IEEE Computer Society, pp. 989-992, IEEE International Symposium on Biomedical Imaging, ISBI 2002, Washington, United States, 7/7/02. https://doi.org/10.1109/ISBI.2002.1029429
Du X, Yang Y, Le Visage C, Chen HH, De Jong R, Qiu B et al. Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. In Proceedings - International Symposium on Biomedical Imaging. Vol. 2002-January. IEEE Computer Society. 2002. p. 989-992. 1029429 https://doi.org/10.1109/ISBI.2002.1029429
Du, Xiangying ; Yang, Yuesong ; Le Visage, Catherine ; Chen, Hunter H. ; De Jong, Robert ; Qiu, Bensheng ; Wang, Danming ; Leong, Kam W. ; Hamper, Ulrike Maria ; Yang, Xiaoming. / Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. Proceedings - International Symposium on Biomedical Imaging. Vol. 2002-January IEEE Computer Society, 2002. pp. 989-992
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