Microscale pressure measurements based on an immiscible fluid/fluid interface

Jing Yang, Xing Duan, Andrew K. Fraser, Mohammad Ikbal Choudhury, Andrew J. Ewald, Rong Li, Sean X. Sun

Research output: Contribution to journalArticlepeer-review

Abstract

A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.

Original languageEnglish (US)
Article number20044
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

ASJC Scopus subject areas

  • General

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