TY - JOUR
T1 - Microscale modification of a cation exchange column procedure for plasma ammonia
AU - Oberholzer, V. G.
AU - Schwarz, K. B.
AU - Smith, C. H.
AU - Dietzler, D. N.
AU - Hanna, T. L.
PY - 1976
Y1 - 1976
N2 - A column cation exchange resin procedure for plasma ammonia was modified to require only 100 μl of plasma per determination. Mean analytical recovery of standard from resin (six samples) was 98% (range, 94-100%) as compared to a mean of 63% (range, 58-70) for nine samples when a batch cation exchange procedure was used. Absorbance was proportional to sample concentration up to 800 μmol/liter. Analytical recovery of standard from plasma (six samples) was complete (mean, 103%; range, 90-113). Thirteen aliquots of a specimen of fresh plasma from a single adult individual gave a mean value of 20 μmol/liter (range, 11-26). The mean plasma venous ammonia concentration for 27 adults was 16 μmol/liter (range, 0-39), and for 15 newborns it was 60 (range, 34-102). Values for capillary plasma measured at the same time were higher, and the authors discuss possible explanations for this. Values are given for infants and children from one month to 14 years of age. Effects of storage time and temperature on plasma ammonia concentration are discussed.
AB - A column cation exchange resin procedure for plasma ammonia was modified to require only 100 μl of plasma per determination. Mean analytical recovery of standard from resin (six samples) was 98% (range, 94-100%) as compared to a mean of 63% (range, 58-70) for nine samples when a batch cation exchange procedure was used. Absorbance was proportional to sample concentration up to 800 μmol/liter. Analytical recovery of standard from plasma (six samples) was complete (mean, 103%; range, 90-113). Thirteen aliquots of a specimen of fresh plasma from a single adult individual gave a mean value of 20 μmol/liter (range, 11-26). The mean plasma venous ammonia concentration for 27 adults was 16 μmol/liter (range, 0-39), and for 15 newborns it was 60 (range, 34-102). Values for capillary plasma measured at the same time were higher, and the authors discuss possible explanations for this. Values are given for infants and children from one month to 14 years of age. Effects of storage time and temperature on plasma ammonia concentration are discussed.
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U2 - 10.1093/clinchem/22.12.1976
DO - 10.1093/clinchem/22.12.1976
M3 - Article
C2 - 1000795
AN - SCOPUS:0017158817
VL - 22
SP - 1976
EP - 1981
JO - Advances in Water Resources
JF - Advances in Water Resources
SN - 0309-1708
IS - 12
ER -