Microsatellite primers for Australian and New Guinean pythons isolated with an efficient marker development method for related species

Philip Jordan, Amanda E. Goodman, Stephen Donnellan

Research output: Contribution to journalArticle

Abstract

Microsatellites are powerful molecular genetic markers for many evolutionary and biotechnological investigations, however, development of sufficient microsatellite markers is time-consuming and expensive especially considering the vast numbers of species for which they could be used. In light of the conservative nature of microsatellite loci between related species we describe an alternative approach to microsatellite development. A single round of microsatellite isolation enabled the characterization of sufficient loci for a large number of related python species. From 21 loci isolated in the focal species, an average of 86.2% were conserved within the other species while an average of 60.5% were polymorphic in all 13 python species analysed. Our approach will decrease significantly the expense and time required for microsatellite development for large numbers of related species.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalMolecular Ecology Notes
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Boidae
Python
Microsatellite Repeats
microsatellite repeats
loci
methodology
Genetic Markers
molecular genetics
genetic marker
method
marker
Molecular Biology
genetic markers

Keywords

  • DNA fingerprinting
  • Microsatellite
  • Python
  • Snake
  • Wildlife management

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Microsatellite primers for Australian and New Guinean pythons isolated with an efficient marker development method for related species. / Jordan, Philip; Goodman, Amanda E.; Donnellan, Stephen.

In: Molecular Ecology Notes, Vol. 2, No. 1, 01.01.2002, p. 78-82.

Research output: Contribution to journalArticle

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