Microsatellite DNA polymorphism and heterozygosity among field and laboratory populations of Anopheles gambiae s.s. (Diptera: Culicidae)

Douglas E. Norris, Amy C. Shurtleff, Yeya T. Touré, Gregory C. Lanzako

Research output: Contribution to journalArticlepeer-review

Abstract

We compared microsatellite polymorphism at nine loci located on chromosome 3 among two colonies and a field population of Anopheles gambiae sensu stricto Giles mosquitoes. Numbers of microsatellite alleles observed at each locus and mean heterozygosities were drastically reduced among laboratory colonies. Genetic analysis of the field population used in this study revealed an unprecedented frequency of rare alleles (<0.05). In contrast, colony samples revealed large numbers of alleles with frequencies >0.50. Partitioning of field data to assess the impact of rare alleles, null alleles, and sample size on estimates of mean heterozygosity revealed the plasticity of this measurement and suggests that heterozygosity may be reliably estimated from relatively small collections using microsatellites.

Original languageEnglish (US)
Pages (from-to)336-340
Number of pages5
JournalJournal of medical entomology
Volume38
Issue number2
DOIs
StatePublished - Mar 2001

Keywords

  • Anopheles gambiae
  • Heterozygosity
  • Microsatellite

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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