Abstract
The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (≤35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of ± 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.
Original language | English (US) |
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Pages (from-to) | 1742-1749 |
Number of pages | 8 |
Journal | ELECTROPHORESIS |
Volume | 18 |
Issue number | 10 |
DOIs | |
State | Published - 1997 |
Keywords
- Cancer diagnosis
- Capillary electrophoresis
- Energy-transfer primers
- Microsatellites
- Short tandem repeat
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry