Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers

Yiwen Wang, Su Chun Hung, Jürgen F. Linn, Gabriel Steiner, Alexander N. Glazer, David Sidransky, Richard A. Mathies

Research output: Contribution to journalArticlepeer-review

Abstract

The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (≤35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of ± 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.

Original languageEnglish (US)
Pages (from-to)1742-1749
Number of pages8
JournalELECTROPHORESIS
Volume18
Issue number10
DOIs
StatePublished - Nov 1 1997

Keywords

  • Cancer diagnosis
  • Capillary electrophoresis
  • Energy-transfer primers
  • Microsatellites
  • Short tandem repeat

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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