MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.

Seth Blackshaw, Winston P. Kuo, Peter J. Park, Motokazu Tsujikawa, Jenny M. Gunnersen, Hamish S. Scott, Wee Ming Boon, Seong Seng Tan, Constance L. Cepko

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. RESULTS: We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals. CONCLUSIONS: MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.

Original languageEnglish (US)
Pages (from-to)R17
JournalGenome biology
Volume4
Issue number3
StatePublished - 2003
Externally publishedYes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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