Abstract
In this study, we used ischemia-induced retinal neovascularization (NV) as a model to investigate the possible role of microRNAs in a clinically important disease process. Microarray analysis demonstrated seven microRNAs (miR-106a, -146, -181, -199a, -214, -424, and -451) that were substantially increased and three microRNAs (miR-31, -150, and -184) that were substantially decreased in ischemic retina. Potential targets for the upregulated microRNAs were not identified, but bioinformatic analysis suggested target genes for the downregulated microRNAs, and these were confirmed using a luciferase reporter assay. Real-time reverse transcriptase PCR confirmed that the substantial levels of miR-31, -150, and -184 present in normal retina were significantly reduced in ischemic retina. Interestingly, constitutive levels of miR-31 and -184 are high in the cornea and lens, two avascular tissues. Intraocular injection of pre-miR-31, -150, or -184 significantly reduced ischemia-induced retinal NV, and injection of pre-miR-31 or -150 also significantly reduced choroidal NV. These data suggest that alteration of microRNA levels contributes to two types of ocular NV, and that injection or enhanced expression of microRNAs is a potential therapeutic strategy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1208-1216 |
| Number of pages | 9 |
| Journal | Molecular Therapy |
| Volume | 16 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2008 |
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ASJC Scopus subject areas
- Molecular Biology
Cite this
MicroRNAs regulate ocular neovascularization. / Shen, Jikui; Yang, Xiaoru; Xie, Bing; Chen, Yangjian; Swaim, Mara; Hackett, Sean F.; Campochiaro, Peter A.
In: Molecular Therapy, Vol. 16, No. 7, 07.2008, p. 1208-1216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNAs regulate ocular neovascularization
AU - Shen, Jikui
AU - Yang, Xiaoru
AU - Xie, Bing
AU - Chen, Yangjian
AU - Swaim, Mara
AU - Hackett, Sean F.
AU - Campochiaro, Peter A
PY - 2008/7
Y1 - 2008/7
N2 - In this study, we used ischemia-induced retinal neovascularization (NV) as a model to investigate the possible role of microRNAs in a clinically important disease process. Microarray analysis demonstrated seven microRNAs (miR-106a, -146, -181, -199a, -214, -424, and -451) that were substantially increased and three microRNAs (miR-31, -150, and -184) that were substantially decreased in ischemic retina. Potential targets for the upregulated microRNAs were not identified, but bioinformatic analysis suggested target genes for the downregulated microRNAs, and these were confirmed using a luciferase reporter assay. Real-time reverse transcriptase PCR confirmed that the substantial levels of miR-31, -150, and -184 present in normal retina were significantly reduced in ischemic retina. Interestingly, constitutive levels of miR-31 and -184 are high in the cornea and lens, two avascular tissues. Intraocular injection of pre-miR-31, -150, or -184 significantly reduced ischemia-induced retinal NV, and injection of pre-miR-31 or -150 also significantly reduced choroidal NV. These data suggest that alteration of microRNA levels contributes to two types of ocular NV, and that injection or enhanced expression of microRNAs is a potential therapeutic strategy.
AB - In this study, we used ischemia-induced retinal neovascularization (NV) as a model to investigate the possible role of microRNAs in a clinically important disease process. Microarray analysis demonstrated seven microRNAs (miR-106a, -146, -181, -199a, -214, -424, and -451) that were substantially increased and three microRNAs (miR-31, -150, and -184) that were substantially decreased in ischemic retina. Potential targets for the upregulated microRNAs were not identified, but bioinformatic analysis suggested target genes for the downregulated microRNAs, and these were confirmed using a luciferase reporter assay. Real-time reverse transcriptase PCR confirmed that the substantial levels of miR-31, -150, and -184 present in normal retina were significantly reduced in ischemic retina. Interestingly, constitutive levels of miR-31 and -184 are high in the cornea and lens, two avascular tissues. Intraocular injection of pre-miR-31, -150, or -184 significantly reduced ischemia-induced retinal NV, and injection of pre-miR-31 or -150 also significantly reduced choroidal NV. These data suggest that alteration of microRNA levels contributes to two types of ocular NV, and that injection or enhanced expression of microRNAs is a potential therapeutic strategy.
UR - http://www.scopus.com/inward/record.url?scp=45749088930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45749088930&partnerID=8YFLogxK
U2 - 10.1038/mt.2008.104
DO - 10.1038/mt.2008.104
M3 - Article
C2 - 18500251
AN - SCOPUS:45749088930
VL - 16
SP - 1208
EP - 1216
JO - Molecular Therapy
JF - Molecular Therapy
SN - 1525-0016
IS - 7
ER -