MicroRNA profiling in intraocular medulloepitheliomas

Deepak P. Edward, Hind Alkatan, Qundeel Rafiq, Charles G Eberhart, Saleh Al Mesfer, Nicola Ghazi, Leen Al Safieh, Altaf A. Kondkar, Khaled K. Abu Amero

Research output: Contribution to journalArticle

Abstract

Purpose: To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT). Material and Methods: Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body controls (n=8). The clinical history and phenotype was recorded. MiRNA profiles were determined using the Affymetrix GeneChip miRNA Arrays analyzed using expression console 1.3 software. Validation of significantly dysregulated miRNA was confimed by quantitaive real-time PCR. The web-based DNA Intelligent Analysis (DIANA)-miRPath v2.0 was used to perform enrichment analysis of differentially expressed (DE) miRNA gene targets in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Results: The pathologic evaluation revealed one benign (benign non-teratoid, n=1) and six malignant tumors (malignant teratoid, n=2; malignant non-teratoid, n = 4). A total of 88 miRNAs were upregulated and 43 miRNAs were downregulated significantly (P

Original languageEnglish (US)
Article numbere0121706
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 25 2015

Fingerprint

Primitive Neuroectodermal Tumors
MicroRNAs
microRNA
Genes
formalin
alkanes
Encyclopedias
Ciliary Body
quantitative polymerase chain reaction
genes
RNA
phenotype
Paraffin
Formaldehyde
neoplasms
genome
Tumors
Real-Time Polymerase Chain Reaction
DNA
Down-Regulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Edward, D. P., Alkatan, H., Rafiq, Q., Eberhart, C. G., Al Mesfer, S., Ghazi, N., ... Abu Amero, K. K. (2015). MicroRNA profiling in intraocular medulloepitheliomas. PLoS One, 10(3), [e0121706]. https://doi.org/10.1371/journal.pone.0121706

MicroRNA profiling in intraocular medulloepitheliomas. / Edward, Deepak P.; Alkatan, Hind; Rafiq, Qundeel; Eberhart, Charles G; Al Mesfer, Saleh; Ghazi, Nicola; Al Safieh, Leen; Kondkar, Altaf A.; Abu Amero, Khaled K.

In: PLoS One, Vol. 10, No. 3, e0121706, 25.03.2015.

Research output: Contribution to journalArticle

Edward, DP, Alkatan, H, Rafiq, Q, Eberhart, CG, Al Mesfer, S, Ghazi, N, Al Safieh, L, Kondkar, AA & Abu Amero, KK 2015, 'MicroRNA profiling in intraocular medulloepitheliomas', PLoS One, vol. 10, no. 3, e0121706. https://doi.org/10.1371/journal.pone.0121706
Edward DP, Alkatan H, Rafiq Q, Eberhart CG, Al Mesfer S, Ghazi N et al. MicroRNA profiling in intraocular medulloepitheliomas. PLoS One. 2015 Mar 25;10(3). e0121706. https://doi.org/10.1371/journal.pone.0121706
Edward, Deepak P. ; Alkatan, Hind ; Rafiq, Qundeel ; Eberhart, Charles G ; Al Mesfer, Saleh ; Ghazi, Nicola ; Al Safieh, Leen ; Kondkar, Altaf A. ; Abu Amero, Khaled K. / MicroRNA profiling in intraocular medulloepitheliomas. In: PLoS One. 2015 ; Vol. 10, No. 3.
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