MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma

Ming Yuan, Ana Cristina A.L. Da Silva, Antje Arnold, Laurence Okeke, Heather Ames, Lina S. Correa-Cerro, M. Adelita Vizcaino, Cheng Ying Ho, Charles G Eberhart, Fausto J Rodriguez

Research output: Contribution to journalArticle

Abstract

Members of the miR-125 family are strongly expressed in several tissues, particularly brain, but may be dysregulated in cancer including adult and pediatric glioma. In this study, miR-125 members were downregulated in pilocytic astrocytoma (PA) as a group compared to non-neoplastic brain in the Agilent platform. In the Nanostring platform, miR-125 members were downregulated primarily in pleomorphic xanthoastrocytomas and gangliogliomas. Using CISH for miR-125b, highest levels of expression were present in grade II tumors (11/33, 33% grade II tumors with 3+ expression compared to 3/70, 4% grade I tumors) (p < 0.001). When focusing on the two histologic subgroups with the largest number of samples, PA and diffuse astrocytoma (DA), the highest expression levels were present in DA, in comparison with the PA group (p = 0.01). Overexpression of miR-125b in pediatric low grade glioma (PLGG) derived cell lines (Res186, Res259, and BT66) resulted in decreased growth and invasion, as well as apoptosis. Additionally, miR-125b overexpression in BT66 resulted in senescence. These findings suggest that miR-125 is frequently underexpressed in PLGG, and overexpression results in a decrease in cell growth and induction of apoptosis, findings that deserve further investigation given its potential as a novel therapeutic strategy for PLGG.

Original languageEnglish (US)
Article number12506
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

MicroRNAs
Glioma
Astrocytoma
Pediatrics
Growth
Neoplasms
Down-Regulation
Ganglioglioma
Apoptosis
Brain
Cell Line

ASJC Scopus subject areas

  • General

Cite this

MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma. / Yuan, Ming; Da Silva, Ana Cristina A.L.; Arnold, Antje; Okeke, Laurence; Ames, Heather; Correa-Cerro, Lina S.; Vizcaino, M. Adelita; Ho, Cheng Ying; Eberhart, Charles G; Rodriguez, Fausto J.

In: Scientific Reports, Vol. 8, No. 1, 12506, 01.12.2018.

Research output: Contribution to journalArticle

Yuan, M, Da Silva, ACAL, Arnold, A, Okeke, L, Ames, H, Correa-Cerro, LS, Vizcaino, MA, Ho, CY, Eberhart, CG & Rodriguez, FJ 2018, 'MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma', Scientific Reports, vol. 8, no. 1, 12506. https://doi.org/10.1038/s41598-018-30942-4
Yuan, Ming ; Da Silva, Ana Cristina A.L. ; Arnold, Antje ; Okeke, Laurence ; Ames, Heather ; Correa-Cerro, Lina S. ; Vizcaino, M. Adelita ; Ho, Cheng Ying ; Eberhart, Charles G ; Rodriguez, Fausto J. / MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
@article{830911957c644900b54513d9607f3ba9,
title = "MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma",
abstract = "Members of the miR-125 family are strongly expressed in several tissues, particularly brain, but may be dysregulated in cancer including adult and pediatric glioma. In this study, miR-125 members were downregulated in pilocytic astrocytoma (PA) as a group compared to non-neoplastic brain in the Agilent platform. In the Nanostring platform, miR-125 members were downregulated primarily in pleomorphic xanthoastrocytomas and gangliogliomas. Using CISH for miR-125b, highest levels of expression were present in grade II tumors (11/33, 33{\%} grade II tumors with 3+ expression compared to 3/70, 4{\%} grade I tumors) (p < 0.001). When focusing on the two histologic subgroups with the largest number of samples, PA and diffuse astrocytoma (DA), the highest expression levels were present in DA, in comparison with the PA group (p = 0.01). Overexpression of miR-125b in pediatric low grade glioma (PLGG) derived cell lines (Res186, Res259, and BT66) resulted in decreased growth and invasion, as well as apoptosis. Additionally, miR-125b overexpression in BT66 resulted in senescence. These findings suggest that miR-125 is frequently underexpressed in PLGG, and overexpression results in a decrease in cell growth and induction of apoptosis, findings that deserve further investigation given its potential as a novel therapeutic strategy for PLGG.",
author = "Ming Yuan and {Da Silva}, {Ana Cristina A.L.} and Antje Arnold and Laurence Okeke and Heather Ames and Correa-Cerro, {Lina S.} and Vizcaino, {M. Adelita} and Ho, {Cheng Ying} and Eberhart, {Charles G} and Rodriguez, {Fausto J}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-30942-4",
language = "English (US)",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma

AU - Yuan, Ming

AU - Da Silva, Ana Cristina A.L.

AU - Arnold, Antje

AU - Okeke, Laurence

AU - Ames, Heather

AU - Correa-Cerro, Lina S.

AU - Vizcaino, M. Adelita

AU - Ho, Cheng Ying

AU - Eberhart, Charles G

AU - Rodriguez, Fausto J

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Members of the miR-125 family are strongly expressed in several tissues, particularly brain, but may be dysregulated in cancer including adult and pediatric glioma. In this study, miR-125 members were downregulated in pilocytic astrocytoma (PA) as a group compared to non-neoplastic brain in the Agilent platform. In the Nanostring platform, miR-125 members were downregulated primarily in pleomorphic xanthoastrocytomas and gangliogliomas. Using CISH for miR-125b, highest levels of expression were present in grade II tumors (11/33, 33% grade II tumors with 3+ expression compared to 3/70, 4% grade I tumors) (p < 0.001). When focusing on the two histologic subgroups with the largest number of samples, PA and diffuse astrocytoma (DA), the highest expression levels were present in DA, in comparison with the PA group (p = 0.01). Overexpression of miR-125b in pediatric low grade glioma (PLGG) derived cell lines (Res186, Res259, and BT66) resulted in decreased growth and invasion, as well as apoptosis. Additionally, miR-125b overexpression in BT66 resulted in senescence. These findings suggest that miR-125 is frequently underexpressed in PLGG, and overexpression results in a decrease in cell growth and induction of apoptosis, findings that deserve further investigation given its potential as a novel therapeutic strategy for PLGG.

AB - Members of the miR-125 family are strongly expressed in several tissues, particularly brain, but may be dysregulated in cancer including adult and pediatric glioma. In this study, miR-125 members were downregulated in pilocytic astrocytoma (PA) as a group compared to non-neoplastic brain in the Agilent platform. In the Nanostring platform, miR-125 members were downregulated primarily in pleomorphic xanthoastrocytomas and gangliogliomas. Using CISH for miR-125b, highest levels of expression were present in grade II tumors (11/33, 33% grade II tumors with 3+ expression compared to 3/70, 4% grade I tumors) (p < 0.001). When focusing on the two histologic subgroups with the largest number of samples, PA and diffuse astrocytoma (DA), the highest expression levels were present in DA, in comparison with the PA group (p = 0.01). Overexpression of miR-125b in pediatric low grade glioma (PLGG) derived cell lines (Res186, Res259, and BT66) resulted in decreased growth and invasion, as well as apoptosis. Additionally, miR-125b overexpression in BT66 resulted in senescence. These findings suggest that miR-125 is frequently underexpressed in PLGG, and overexpression results in a decrease in cell growth and induction of apoptosis, findings that deserve further investigation given its potential as a novel therapeutic strategy for PLGG.

UR - http://www.scopus.com/inward/record.url?scp=85051986726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051986726&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-30942-4

DO - 10.1038/s41598-018-30942-4

M3 - Article

C2 - 30131528

AN - SCOPUS:85051986726

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 12506

ER -