MicroRNA let-7e is associated with the pathogenesis of experimental autoimmune encephalomyelitis

Hongbing Guan, Daping Fan, Davit Mrelashvili, Haiping Hao, Narendra P. Singh, Udai P. Singh, Prakash S. Nagarkatti, Mitzi Nagarkatti

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) play important roles in the regulation of immune responses. There is evidence that miRNAs also participate in the pathogenesis of multiple sclerosis (MS), but how the miRNAs regulate the pathogenesis of MS is still under investigation. The identification of new members of the miRNA family associated with the pathogenesis of MS could facilitate early diagnosis and treatment. Here, we show that the level of miRNA let-7e is significantly upregulated in EAE, an animal model of MS using miRNA array and quantitative real-time PCR. The expression of let-7e was mainly in CD4+ T cells and infiltrated mononuclear cells of CNS, and highly correlated with the development of EAE. We found that let-7e silencing in vivo inhibited encephalitogenic Th1 and Th17 cells and attenuated EAE, with reciprocal increase of Th2 cells; overexpression of let-7e enhanced Th1 and Th17 cells and aggravated EAE. We also identified IL-10 as one of the functional targets of let-7e. Together, we propose that let-7e is a new miRNA involved in the regulation of encephalitogenic T-cell differentiation and the pathogenesis of EAE.

Original languageEnglish (US)
Pages (from-to)104-114
Number of pages11
JournalEuropean Journal of Immunology
Volume43
Issue number1
DOIs
StatePublished - Jan 1 2013

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Autoimmune Experimental Encephalomyelitis
MicroRNAs
Multiple Sclerosis
Th17 Cells
Th1 Cells
T-Lymphocytes
Th2 Cells
Interleukin-10
Real-Time Polymerase Chain Reaction
Early Diagnosis
Cell Differentiation
Animal Models

Keywords

  • Cell differentiation
  • EAE/MS
  • MicroRNA
  • MiR-let-7e
  • Th1/Th2 cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

MicroRNA let-7e is associated with the pathogenesis of experimental autoimmune encephalomyelitis. / Guan, Hongbing; Fan, Daping; Mrelashvili, Davit; Hao, Haiping; Singh, Narendra P.; Singh, Udai P.; Nagarkatti, Prakash S.; Nagarkatti, Mitzi.

In: European Journal of Immunology, Vol. 43, No. 1, 01.01.2013, p. 104-114.

Research output: Contribution to journalArticle

Guan, H, Fan, D, Mrelashvili, D, Hao, H, Singh, NP, Singh, UP, Nagarkatti, PS & Nagarkatti, M 2013, 'MicroRNA let-7e is associated with the pathogenesis of experimental autoimmune encephalomyelitis', European Journal of Immunology, vol. 43, no. 1, pp. 104-114. https://doi.org/10.1002/eji.201242702
Guan, Hongbing ; Fan, Daping ; Mrelashvili, Davit ; Hao, Haiping ; Singh, Narendra P. ; Singh, Udai P. ; Nagarkatti, Prakash S. ; Nagarkatti, Mitzi. / MicroRNA let-7e is associated with the pathogenesis of experimental autoimmune encephalomyelitis. In: European Journal of Immunology. 2013 ; Vol. 43, No. 1. pp. 104-114.
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