Abstract
BMP4 has been shown to induce C3H10T1/2 pluripotent stem cells to commit to adipocyte lineage. In addition to several proteins identified, microRNAs also play a critical role in the process. In this study, we identified microRNA-140 (miR-140) as a direct downstream component of the BMP4 signaling pathway during the commitment of C3H10T1/2 cells to adipocyte lineage. Overexpression of miR-140 in C3H10T1/2 cells promoted commitment, whereas knockdown of its expression led to impairment. Additional studies indicated that Ostm1 is a bona fide target of miR-140, which is significantly decreased during commitment, and Ostm1 was also demonstrated to function as an anti-adipogenic factor.
Original language | English (US) |
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Pages (from-to) | 8222-8230 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 12 |
DOIs | |
State | Published - Mar 22 2013 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology