MicroR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages

Guangxian Xu; Zhaobo Zhang; Jun Wei; Yilin Zhang; Ying Zhang; Le Guo; Xiaoming Liu

doi:10.1016/j.tube.2013.08.006

MicroR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages

Guangxian Xu, Zhaobo Zhang, Jun Wei, Yilin Zhang, Ying Zhang, Le Guo, Xiaoming Liu

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.

Original language	English (US)
Pages (from-to)	606-611
Number of pages	6
Journal	Tuberculosis
Volume	93
Issue number	6
DOIs	https://doi.org/10.1016/j.tube.2013.08.006
State	Published - Nov 2013

Keywords

BCG
IRAK-1
Macrophage
Mycobacterium
microRNA

ASJC Scopus subject areas

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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@article{a88200c58c264a73997aa93eee7ae342,

title = "MicroR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages",

abstract = "MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.",

keywords = "BCG, IRAK-1, Macrophage, Mycobacterium, microRNA",

author = "Guangxian Xu and Zhaobo Zhang and Jun Wei and Yilin Zhang and Ying Zhang and Le Guo and Xiaoming Liu",

note = "Funding Information: This work was supported by grants from Natural Science Foundation of China (Nos. 31160494 , 30960275 ), the program for New Century Excellent Talents in University (No. NCET 11-11023 ), Postdoctoral Science Foundation of China (No. 20110491554 ), Postdoctoral Special Science Foundation of China (No. 2012T50834 ), and “Doctorate construction disciplines” open project of Ningxia Medical University (No. KF2010-41 ). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2013",

month = nov,

doi = "10.1016/j.tube.2013.08.006",

language = "English (US)",

volume = "93",

pages = "606--611",

journal = "Tuberculosis",

issn = "1472-9792",

publisher = "Churchill Livingstone",

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AU - Xu, Guangxian

AU - Zhang, Zhaobo

AU - Wei, Jun

AU - Zhang, Yilin

AU - Zhang, Ying

AU - Guo, Le

AU - Liu, Xiaoming

N1 - Funding Information: This work was supported by grants from Natural Science Foundation of China (Nos. 31160494 , 30960275 ), the program for New Century Excellent Talents in University (No. NCET 11-11023 ), Postdoctoral Science Foundation of China (No. 20110491554 ), Postdoctoral Special Science Foundation of China (No. 2012T50834 ), and “Doctorate construction disciplines” open project of Ningxia Medical University (No. KF2010-41 ). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2013/11

Y1 - 2013/11

N2 - MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.

AB - MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.

KW - BCG

KW - IRAK-1

KW - Macrophage

KW - Mycobacterium

KW - microRNA

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MicroR-142-3p down-regulates IRAK-1 in response to Mycobacterium bovis BCG infection in macrophages

Abstract

Keywords

ASJC Scopus subject areas

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