Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

Minghan Chen, Natalya V. Zaytseva, Qi Wu, Min Li, Ye Fang

Research output: Contribution to journalArticle

Abstract

We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

Original languageEnglish (US)
Article number193702
JournalApplied Physics Letters
Volume102
Issue number19
DOIs
StatePublished - May 13 2013

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ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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