Micropipette Aspiration of Oocytes to Assess Cortical Tension

Janice P. Evans, Douglas N. Robinson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Just as it is important to understand the cell biology of signaling pathways, it is valuable also to understand mechanical forces in cells. The field of mechanobiology has a rich history, including study of cellular mechanics during mitosis and meiosis in echinoderm oocytes and zygotes dating back to the 1930s. This chapter addresses the use of micropipette aspiration (MPA) to assess cellular mechanics, specifically cortical tension, in mammalian oocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-171
Number of pages9
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1818
ISSN (Print)1064-3745

Keywords

  • Cellular mechanics
  • Meiosis
  • Mitosis
  • Signaling pathways

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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