Micropipette aspiration for studying cellular mechanosensory responses and mechanics

Yee Seir Kee, Douglas N. Robinson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Micropipette aspiration (MPA) is a widely applied method for studying cortical tension and deformability. Based on simple hydrostatic principles, this assay allows the application of a specific magnitude of mechanical stress on cells. This powerful method has revealed insights about cell mechanics and mechanosensing, not only in Dictyostelium discoideum but also in other cell types. In this chapter, we present how to set up a micropipette aspiration system and the experimental procedures for determining cortical tension and mechanosensory responses.

Original languageEnglish (US)
Title of host publicationDictyostelium discoideum Protocols
PublisherHumana Press Inc.
Pages367-382
Number of pages16
ISBN (Print)9781627033015
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume983
ISSN (Print)1064-3745

Keywords

  • Cell mechanics
  • Cortical tension
  • Mechanosensing
  • Mechanosensory response
  • Micropipette aspiration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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