Microgrooved surface modulates neuron differentiation in human embryonic stem cells

David Lu; Chi Shuo Chen; Chao Sung Lai; Sushant Soni; Taranze Lam; Clarence Le; E. Y. Chen; Thien Nguyen; Wei Chun Chin

doi:10.1007/7651_2014_81

Microgrooved surface modulates neuron differentiation in human embryonic stem cells

David Lu, Chi Shuo Chen, Chao Sung Lai, Sushant Soni, Taranze Lam, Clarence Le, E. Y. Chen, Thien Nguyen, Wei Chun Chin

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.

Original language	English (US)
Pages (from-to)	281-287
Number of pages	7
Journal	Methods in Molecular Biology
Volume	1307
DOIs	https://doi.org/10.1007/7651_2014_81
State	Published - 2014

Keywords

Differentiation
HESC
Microgrooves
Neuron
PDMS
Stem cell

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/7651_2014_81

Cite this

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title = "Microgrooved surface modulates neuron differentiation in human embryonic stem cells",

abstract = "Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.",

keywords = "Differentiation, HESC, Microgrooves, Neuron, PDMS, Stem cell",

author = "David Lu and Chen, {Chi Shuo} and Lai, {Chao Sung} and Sushant Soni and Taranze Lam and Clarence Le and Chen, {E. Y.} and Thien Nguyen and Chin, {Wei Chun}",

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AU - Lu, David

AU - Chen, Chi Shuo

AU - Lai, Chao Sung

AU - Soni, Sushant

AU - Lam, Taranze

AU - Le, Clarence

AU - Chen, E. Y.

AU - Nguyen, Thien

AU - Chin, Wei Chun

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.

AB - Stem cell-based therapies have drawn intensive attention in the neuronal regenerative fields. Several studies have revealed that stem cells can serve as an inexhaustible source for neurons for transplantation therapies. However, generation of neurons and directionality has not yet been fully investigated. Herein, we investigate the mechanical ramifications of surface topography on human embryonic cell differentiation. Microgrooved surfaces with various pitches were applied to modulate the neuron differentiation. Our protocol showed that neuron differentiation increased as grove pitch decreased. The results indicated that 2 μm microgrooves can improve neuron growth by ~1.7-fold. Our results indicate the importance of mechanotransduction on neuronal differentiation and highlight the feasibility of manipulating the neuronal differentiation with surface topography, providing new perspectives for accommodating clinical transplantation.

KW - Differentiation

KW - HESC

KW - Microgrooves

KW - Neuron

KW - PDMS

KW - Stem cell

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Microgrooved surface modulates neuron differentiation in human embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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